جامعة أم القرى
قائمة الأبحاث المنشورة 1433- 1434
1. Abdelhafez AM and El-Madbuly MA (2013). Factors Associated with Inadequate Dietary Iron Intake among University Female Students in Makkah, Saudi Arabia. Journal of American Science, 9 (2):141- 149. (Non-ISI)
2. Abdelhafez AM and Al-Mashi SSM (2013). Prevalence of Obesity and Some Related Attributes among Umm Al-Qura University Female Students in Makkah, Saudi Arabia. Pakistan Journal of Nutrition, 12: 275-284. (ISI; No IF)
3. Bukhari SZ, Qazi JI, Ashshi AM, and Zia N (2012) Human immunodeficiency virus type-1 (HIV-1) disease progression and viral activity: a seroepidemiological and molecular study. Journal of the College of Physicians and Surgeons--Pakistan: JCPSP; 22: 565-569. (ISI; IF = 0.3)
4. Ashshi A, Faidah H, Saati A, Abou Elella GA, Alghamdi A, and Mohamed A (2013). Urinary Tract Infections in Pregnant Women, Assessment of Associated Risk Factors in Makkah, KSA. BIOSCIENCES BIOTECHNOLOGY RESEARCH ASIA; 10: 1-8. (Non-ISI)
5. Faidah H, Ashshi A, Abou Elella G, Alghamdi A, and Mohamed A (2013). Urinary Tract Infections among Pregnant Women in Makkah, Saudi Arabi. Biomedical & Pharmacology Journal; 6: 01-07. (Non-ISI)
6. Abou Elella GA, Mohamed A, and Aamer A (2013) Prevalence of Enteropathogens Associated with Neonatal Calf Scour in Cattle and Buffalo Calves using (FASTEST® STRIPS) Rapid Field Test. Assiut Vet. Med. J; 59: 19-26. (Non-ISI)
7. Azzeh, FS (2013). Synergistic Effect of Green Tea, Cinnamon and Ginger Combination on Enhancing Postprandial Blood Glucose. Pakistan Journal of Biological Sciences, 16(2): 74-79. (ISI; No IF)
8. Baddour AA and Saleh HA (2013). Use Six Sigma Approach to Improve Healthcare Workers Safety. International Journal of Pure Applied Sciences and Technology; 18: 54-68. (Non-ISI)
9. Omar OAS, Bukhari HM, ElSawy NA, and Header EA (2013) Efficacy of Capsicum Frutescens in Curing the Peptic Ulcer. Int. J. Pure Appl. Sci. Technol; 15: 43-54. (Non-ISI; Cited 1)
10. El-Sebaei M, El-Ashker M, and El-Boshy M (2013). The role of acute phase cytokines in the recovery and disease progress of Theileria annulata-infected cattle. Comparative Clinical Pathology;1-6. (ISI; No IF; cited 1)
11. Abdalla OA, EL-Boshy ME, Hamid FMA, and Ali NM (2013). Clinicopathological studies of dietary supplementation of Saccharomyces cerevisiae in calves. Journal of American Science; 9. (Non-ISI)
12. Abdalla OA, EL-Boshy ME, El-Khodary SA, Reisha EF and Gadalla HA (2013). Selective Serum Oxidant, Antioxidant and Trace Elements Profile in Ossimi Sheep Affected with Pregnancy Toxemia. Life Science Journal; 10:2833-2837. (Non-ISI)
13. El-Boshy ME, Abdalla OM, Risha A, and Moustafa F (2013). Effect of Withania somnifera Extracts on Some Selective Biochemical, Hematological, and Immunological Parameters in Guinea Pigs Experimental Infected with E. coli. ISRN veterinary science; 2013:153427. (Non-ISI, Cited 1)
14. Al-Kushi AG, El-Boshy ME, ElSawy NA, Omar OSO, Header EA (2013). Pathological Comparative Studies on Aqueous and Ethanolic Extracts of Zingiber officinale on Antioxidants and Hypolipidemic Effects in Rats. Life Science Journal; 10: 2393-2403. (Non-ISI)
15. El-Boshy M and Abdall O (2013) Effect of lactoferrin on some selective immunological parameters in rats immunosuppressed by cyclophosphamide. Journal of Investigational Biochemistry; 2: 136-140. (Non-ISI; Cited 1)
16. El-Ashker M, Salama M, and El-Boshy M (2013). Traumatic reticuloperitonitis in water Buffalo (Bubalus bubalis): clinical findings and the associated inflammatory response. Journal of Veterinary Medicine; 2013. (Non-ISI; Cited 1)
17. Elgazar AF, Rezq AA and Bukhari HM (2013). Anti-Hyperglycemic Effect of Saffron Extract in Alloxan-Induced Diabetic Rats. European Journal of Biological Sciences 5 (1): 14-22. (Non-ISI; Cited 3).
18. AbdulJawad SA, Hasanein MA, Header EA (2013). Influence of Aqueous Extract of Red Chillis Pepper as Curative for Gastric Ulcer in Albino Rats. Journal of Natural Sciences Research, 3(4). (Non-ISI; Cited 1)
19. Kensara OA (2013). Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats, International Journal of Nutrition and Metabolism., 5 (4): 61-68. (Non-ISI)
20. Kensara OA, ElSawy NA, El-Shemi AG, and Header EA (2013). Thymus vulgaris supplementation attenuates blood pressure and aorta damage in hypertensive rats. Journal of Medicinal Plants Research; 7: 669-676. (Non-ISI; Cited 1)
21. Khan MA, Ashshi A, and Mahomed M (2012) Drug resistance patterns of acinetobacter baumannii in makkah, saudi arabia. Pakistan Journal of Medical Research; 51 (4):127. (Non-ISI)
22. Johargy AK, Mahomed MF, Khan MM, and Kabrah S (2013) Anti-hepatitis E virus seropositivity in a group of male blood donors in Makkah, Saudi Arabia. Journal of Pakistan Medical Association; 63: 185-189. (Non-ISI; Cited 3)
23. Saleh HA and Khereldeen MM (2013) Physicians’ Perception towards Patients’ Rights in Two Governmental Hospitals in Mecca, KSA. Int. J. Pure Appl. Sci. Technol; 17: 37-47. (Non ISI; Cited 1)
24. Shaikh-Omar OA, Header EA and Bukhari HM (2013). Digestive Discomforts and Effect of Ingested Food in People Performing Hajj. Journal of Basic & Applied Sciences; 9: 341-347. (Non-ISI)
25. Elkamel AA and Mohamed AM (2012). Differential Identification of Flavobacterium Species by Sequence Analysis of Genus-Specific Hypervariable 16S-23S rDNA Intergenic Spacer Target. World Journal of Fish and Marine Sciences; 4: 597-603. (ISI; No IF)
26. Mohamed AM, Abou El Ella GA, and Hayder IA (2013) Antioxidant effect of bee pollen on immune status of hyperglycemic rats. Assiut Vet Med J; 59: 107-116. (Non-ISI)
27. Zaglool D, Mohamed A, Khodari Y, and Farooq M (2013) Crypto-Giardia antigen rapid test versus conventional modified Ziehl-Neelsen acid fast staining method for diagnosis of cryptosporidiosis. Asian Pacific journal of tropical medicine; 6: 212-215. (ISI; IF = 0.92; Cited 1)
28. El-Ashmony SMA, Morsi HK, and Abdelhafez AM (2012). Effect of Zinc Supplementation on Glycemic Control, Lipid Profile, and Renal Functions in Patients with Type II Diabetes: A Single Blinded, Randomized, Placebo-Controlled, Trial. Journal of Biology, Agriculture and Healthcare; 2: 33-41. (Non-ISI; Cited 3)
29. Zalata AA, Morsy HK, Badawy AE-N, Elhanbly S, and Mostafa T (2012). ACE gene insertion/deletion polymorphism seminal associations in infertile men. The Journal of urology; 187: 1776-1780. (ISI; IF= 3.7; Cited 4)
30. Shalaby and Morsy HK (2013). Effect of pregabalin on erectile function and penile NOS expression in rats with streptozotocin-induced diabetes. Exp Clin Endocrinol Diabetes; 121: 230-3. (ISI; IF = 1.76; Cited 1)
31. Osfor M, Kensarah OA, Azzeh FS, Elmadbouly M and El-Qutry M (2013). Biochemical changes in lipid and carbohydrate metabolism in obese diabetic male albino rats. International Journal of Nutrition and Food Sciences; 2: 24-30. (Non-ISI)
32. Osfor MMH, Hegazy A, Abd El-moaty M, Elmadbouly MA, Afify AMR and AElbahnasawy ASM (2013). Hypo-cholesterolemic and hypoglycemic effects of orange albedo powder (citrus aurantium l.) on male albino Rats. International Journal of Nutrition and Food Sciences; 2: 70-76. (Non-ISI)
33. Osfor MMH, Elmadbouly MA, Elsoadaa SS, Metair AH and Hussain AAA (2013). Relation between Hypercholesterolemia and Insulin like growth factor-1 in Elderly Women suffer from Hypothyroidism. Journal of Natural Sciences Research; 3: 160-164. (Non-ISI)
34. Osfor MMH, El-Ashi A, El-Madbouly M, Zaki MS and Negm D (2013). A prospective study of Green Tea and panax Ginseng against mutagens & carcinogens produced during Thermolyzed Meat and Fish. Life Science Journal; 10: 1754-65. (Non-ISI)
35. Saleh HA and Kheeeldeen MM (2013). Physicians’ perception towards Patients’ Rights in tow Governmental Hospitals in Mecca, KSA. International Journal of Pure Applied Sciences and Technology; 17: 37-47. (Non-ISI)
ملخص الأبحاث المنشورة 2012-2013
1. Abdelhafez AM and El-Madbuly MA (2013). ‘Factors Associated with Inadequate Dietary Iron Intake among University Female Students in Makkah, Saudi Arabia.’ Journal of American Science, 9 (2):141- 149.
http://www.jofamericanscience.org/journals/am-sci/am0902/019_15814am0902_141_149.pdf
(Clinical Nutrition Department)
ABSTRACT
Iron deficiency is the most common nutritional problem among females. The aim of the current study was to evaluate the dietary iron intake and the factors influencing it among university female students in Makkah ,Saudi Arabia. A Cross sectional study was conducted on 240 students. Self -administered questionnaire was used to collect demographic, medical, and dietary histories. Weight, height, and Body Mass Index(BMI) were recorded. The Arab Food Analysis Program was used for energy and nutrient analysis. Results showed that (42.5%) of students had inadequate dietary iron intake. The inadequate group was consuming < 80% of the recommendation of almost all macro and micronutrients (P < 0.001). Regression analysis showed that skipping meals, taking phosphorus, and niacin below 80 % Recommended Daily Allowance (RDA )were independently related to inadequate iron intake (P < 0.05). It is advisable to design nutrition educational program to improve students' awareness of this problem.
2. Abdelhafez AM and Al-Mashi SSM (2013). ‘Prevalence of Obesity and Some Related Attributes among Umm Al-Qura University Female Students in Makkah, Saudi Arabia.’ Pakistan Journal of Nutrition, 12: 275-284.
http://www.pjbs.org/pjnonline/fin2605.pdf
(Clinical Nutrition Department)
ABSTRACT
Objective: The prevalence of obesity is increasing worldwide at an alarming rate in both developing and developed countries. It has become a serious epidemic health problem, estimated to be the fifth leading cause of mortality at global level. This study was designed to determine the prevalence of obesity among students at Umm Al-Qura University in Makkah, Saudi Arabia and to investigate some of the epidemiologic risk factors contributing to it. A cross sectional study was conducted during the academic year 2009-2010. The study included 224 randomly selected students enrolled at Umm Al-Qura University. Data were collected using a pretested, structured self-administered questionnaire. Weight, height, waist hip ratio and Body Mass Index (BMI) were recorded for each student. Statistical analysis was done using SPSS version 16. The prevalence of overweight and obesity among the studied students was 25% (19.2%, were overweight and 5.8% were obese). Factors behind overweight and obesity were; high family monthly income, family history of obesity, eating while bored, depressed and upset. The association between physical activity, dietary factors and obesity were not observed in this study. Our findings suggest the need for strategies and coordinated efforts at all levels to reduce the tendency of overweight and obesity and to promote healthy eating habits in our youth.
3. Bukhari SZ, Qazi JI, Ashshi AM, and Zia N (2012). ‘Human immunodeficiency virus type-1 (HIV-1) disease progression and viral activity: a seroepidemiological and molecular study.’ Journal of the College of Physicians and Surgeons--Pakistan: JCPSP; 22: 565-569.
http://jcpsp.pk/archive/2012/Sep2012/05.pdf
(Laboratory Medicine Department)
ABSTRACT
Objective: To determine the frequency and epidemiological characterization of human immunodeficiency virus type-1 (HIV-1) infection, HIV disease progression, immune status and viral activity. Study design: Descriptive study. Place and duration of study: Department of Microbiology, University of the Punjab and Institute of Public Health, Lahore, from September 2005 to August 2008. Methodology: The study enrolled samples from general population, high risk groups and spouses of HIV+ deport workers with criteria; positive double enzyme linked immunosorbent assay (ELISA) and positive western blot. Immune status and viral activity was determined by cluster determinants (CD4+ and CD8+) cell count, ratio of CD4+/CD8+ on flow cytometer, and HIV RNA viral load on polymerase chain reaction (PCR). Results: A total of 116 HIV+ untreated subjects enrolled after screening of 2260 blood samples. The seroprevalence rate in general population, high risk individuals and spouses of HIV+ deport workers was found 0%, 0.4% and 26% respectively. The CD4+ cell count was found 533/mm3 (range 12-1800/mm3) and plasma viral load 27,122 copies/ml (range 00-40,621). The CD4+/CD8+ ratios < 0.5, < 1, < 1.5 and < 2 appeared as 17.2%, 30.2%, 51.7% and 0.9% respectively. Significant correlation was observed between plasma viral load, CD4+ count and CD4+/CD8+ ratio (p = 0.001). CD4+ T-cell counts < 200 cells/mm3 was found in 23 HIV+ patients. Conclusion: There was a low frequency of HIV in the general population and high risks groups as compared to very high frequency in spouses of HIV+ deport workers with significant correlation of viral activity and immune status.
4. Ashshi A, Faidah H, Saati A, Abou Elella GA, Alghamdi A, and Mohamed A (2013). ‘Urinary Tract Infections in Pregnant Women, Assessment of Associated Risk Factors in Makkah, KSA.’ Biosciences Biotechnology Research Asia; 10: 1-8.
(Laboratory Medicine Department)
ABSTRACT
Urinary tract infection represents a serious health problem in pregnant women. Many risk factors could contribute to the occurrence of UTI in pregnant women. The aim of the current study was to assess different risk factors that may influence the infection among pregnant women in Makkah, KSA. A total of 200 pregnant women that visited maternity and children hospital in Makkah were investigated. Personal data as well as medical history and some risk factors data were collected using a well-structured questionnaire. Midstream clean catch urine samples for urinalysis, and urine culture were collected from all investigated cases for diagnosis of UTI. The results revealed the presence of significant association between some investigated risk factors and UTI in pregnant women. The risk factors that were recorded to influence UTI among pregnant women in the current study including advanced age, low educational level, multiparity, as well as unsatisfactory personal hygiene. Moreover, diabetic condition, using IUD as contraceptives and using panties of silky materials are among the influencing factors. In conclusion, extreme care has to be taken by pregnant women in particular and women in general towards personal hygiene, type of contraceptives and type of panties materials. In addition, diabetic condition should be avoided or controlled in order to decrease the
risk of UTI.
5. Faidah H, Ashshi A, Abou Elella G, Alghamdi A, and Mohamed A (2013). ‘Urinary Tract Infections among Pregnant Women in Makkah, Saudi Arabia.’ Biomedical & Pharmacology Journal; 6: 01-07.
(Laboratory Medicine Department)
ABSTRACT
Urinary tract infection represents a serious health problem in pregnant women. Scarce
information is available about the profile of urinary tract infections among pregnant women in
Makkah, KSA. The aim of the current study was to study the prevalence of UTI among pregnant
women in Makkah, KSA and to investigate the most frequent causative agents and drug resistance
profiles associated with such infections. A total of 200 pregnant women that visited Maternity and
Children Hospital in Makkah were investigated. Personal data as well as medical history were
collected using a well-structured questionnaire. Midstream clean catch urine samples for urinalysis,
and urine culture were collected from all investigated cases. The results revealed that 20% of
investigated pregnant women were positive for UTI (12% with symptomatic UTI and 8% were
asymptomatic). Escherichia coli was the most frequently isolated bacterial agent (25%) from both
symptomatic and asymptomatic bacteriuria. Amoxicillin, cefoxitin, celtaxidime, fusidic acid,
norfloxacin, ofloxzcin and penicillin showed the least resistance frequency (2.6%). In conclusion,
high rate of UTI infection (20%) was recorded among pregnant women with E. coli being the most
frequently encountered causative agent. Although symptoms are good markers of UTI during
pregnancy, special care has to be directed towards asymptomatic cases. Finally, Amoxicillin,
cefoxitin, celtaxidime, norflaoacin, penicillin and fusidic acid are the most useful antibiotics for
treatment of UTI as they were able to inhibit most of the currently isolated UTI pathogens.
6. Abou Elella GA, Mohamed A, and Aamer A (2013). ‘Prevalence of Enteropathogens Associated with Neonatal Calf Scour in Cattle and Buffalo Calves using (FASTEST® STRIPS) Rapid Field Test.’ Assiut Vet. Med. J; 59: 19-26.
http://www.aun.edu.eg/journal_files/118_J_6361.pdf
(Laboratory Medicine Department)
ABSTRACT
The prevalence of calf scour-causing enteropathogens was investigated in the current study using rapid field test. Fecal samples were collected from a total of 124 cattle (Bos indicus) and water buffalo (Bubalus bubalis) calves. Investigated calves were divided into 4 age groups (0–4 days old, 5–14 days old, 15–21 days old and >21 days old). Immuno-chromatographic rapid tests (FASTest® Strips) were used for the detection of Bovine coronavirus, rotavirus, Cryptosporidium parvum and E.coli-K99 (F5) from investigated diarrheic calves. In cattle calves (n= 76), C. parvum was the most frequently encountered infection among all age groups followed by rotavirus with an overall rate of 59.2% and 35.5%, respectively. In water buffalo calves (n = 48), the highest infection rate was recorded for C. parvum (39.6%) followed by both rotavirus and E.coli (20.8% each) in all age groups with exception of the first age group (0-4 days-old), where the highest infection rate was recorded as E.coli (57.1%) followed by C. parvum (28.6%). In conclusion, C. parvum was reported as the most frequently encountered causative agent among both cattle and water buffalo calves whilst coronavirus infection seemed to be of minor importance in the investigated population. The higher rate of C. parvum infection was recorded among the second age group (5-14 day old) of cattle calves and the forth age group (> 21 day old) of water buffalo calves, whilst the highest rate of E.coli-K99 (F5) infection was recorded among the first age group (0-4 days) of both cattle and water buffalo calves.
7. Azzeh, FS (2013). ‘Synergistic Effect of Green Tea, Cinnamon and Ginger Combination on Enhancing Postprandial Blood Glucose.’ Pakistan Journal of Biological Sciences, 16: 74-79.
http://www.ncbi.nlm.nih.gov/pubmed/24199490
(Clinical Nutrition Department)
ABSTRACT
This study was maintained to determine the immediate effect of green tea, cinnamon, ginger and combination of them on postprandial glucose levels. The Glycemic Index (GI) for previous treatments was measured as an indicator for postprandial glucose pattern. Twenty-two healthy volunteers from both genders were enrolled in this study. Mean age was 21.3 years and mean BMI was 24.6 kg m(-2). For each herb and combination treatment, a concentration of 2.5% aqueous tea extract was prepared. The GI of green tea, cinnamon and ginger were 79, 63 and 72 respectively. Herbs combination exerted GI of 60, which was the lowest. Combination of these herbs showed the best lowering effect on postprandial glucose levels as compared with each herb alone. A potential synergism from the active ingredients of blended herbs was determined.
8. Baddour AA and Saleh HA (2013). ‘Use Six Sigma Approach to Improve Healthcare Workers Safety.’ International Journal of Pure Applied Sciences and Technology; 18: 54-68.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.401.2339&rep=rep1&type=pdf
(Health Management Department)
ABSTRACT
Health care workers (HCWs) suffer between 600,000 and one million injuries from conventional needles and sharps annually. These exposures can lead to hepatitis B, hepatitis C and Human Immunodeficiency Virus (HIV), the virus that causes AIDS. At least 1,000 Health care workers are estimated to contract serious infections annually from needle stick and sharps injuries. The aim of this study was use of six sigma approach by utilizing the five-step DMAIC process to identify the causes, practice and conditions related needle stick injuries and proposing the appropriate policies and procedures to decrease the incidence of needle stick injuries to improve safety of healthcare workers. This study could serve as valuable serve as valuable models for other safety concerns in the health care workplace.
9. Omar OAS, Bukhari HM, ElSawy NA, and Header EA (2013). ‘Efficacy of Capsicum Frutescens in Curing the Peptic Ulcer.’ Int. J. Pure Appl. Sci. Technol; 15: 43-54.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.301.2104&rep=rep1&type=pdf
(Clinical Nutrition & Laboratory Medicine Departments)
ABSTRACT
Chili peppers are often used around the world to make a wide variety of sauces, known as hot sauce, chili sauce, or pepper sauce. Red chilis contain high amounts of vitamin C and carotene (provitamin A). meanwhile Several studies found that capsaicin could have an anti-ulcer protective effect on stomachs infected with H. pylori by affecting the chemicals the stomach secretes in response to infection. The present study was designated to clear out the effect of aqueous extracts of red chilli pepper (RCP) and some spicy foods on healing acute gastric ulcer induced by aspirin in rats. Sixty six adult male albino rats (170+5g B.Wt.) of Sprague Dawley Strain were obtained from animal house of the faculty of medicine, Um Al Qura University were used and disported into 6 groups (n= 6 rats), one of them used as control -ve while other groups had given aspirin orally (200mg/kg B.Wt.), one of these groups left as control +ve and other groups administrated with aqueous extracts of RCP at doses of 300 and 600 mg/kg B.Wt. and 30% spicy nuggets and chicken for seven days. The length of gastric ulcer, volume of gastric juice, pH value, and histopathological changes were examined. Our results revealed that, oral administration of aspirin (200mg/kg B.Wt.) induced gastric ulcer in rats; the mean length of gastric ulcer in control +ve group was higher compared with in control -ve group. Also, Oral administration of RCP extract at a dose of 600mg/kg B.Wt., caused high decrease in the length of gastric ulcer. On the other hand, the lowest decrease of gastric ulcer length was happened in rats fed on 30% spicy nuggets. The results concluded that oral administration with all tested plants reduced the length of gastric ulcer, volume of gastric juice, and histopathological changes. On the other hand, RCP extracts increased pH value of gastric juice. According to the results, RCP could be used for healing acute gastric ulcer disease.
10. El-Sebaei M, El-Ashker M, and El-Boshy M (2013). ‘The role of acute phase cytokines in the recovery and disease progress of Theileria annulata-infected cattle.’ Comparative Clinical Pathology;1-6.
http://link.springer.com/article/10.1007%2Fs00580-013-1812-7
(Laboratory Medicine Department)
ABSTRACT
The present study was conducted to evaluate the cytokine response following natural infection ofTheileria annulata in cattle. Initial survey included 173 crossbred cattle which were examined for the presence of Theileria piroplasms. The investigated cattle were clinically and parasitologically examined. Blood samples were collected from all examined cattle for microscopic examination, PCR assays (using primers of Theileria spp., Babesia spp., and T. annulata), and cytokines measurement. It was found that 38 cattle were positive for the presence of at least one species ofTheileria; meanwhile Babesia piroplasms were not detected either by microscopy or PCR assay. When T. Annulata-specific primers were used, 33 gave positive results. Twenty-two out of 33 T. annulata-infected cattle were only included in this study together with contemporaneous controls (n = 10). According to the severity of clinical signs, T. annulata-infected cattle were categorized into two groups; group 1 included ten cattle with mild clinical signs and group 2 included 12 cattle with overt clinical signs. Biochemically, tumor necrosis factor alpha, interleukin (IL)-1β, IL-6, IL-12, haptoglobin, and Fb were significantly higher (p < 0.05) in diseased cattle compared to control and in cattle of group 2 compared to those in group 1, while interferon gamma showed no significant variations between the two groups. We conclude that measurements of the pro-inflammatory cytokines following T. annulata infection provide a valuable and quantitative assessment of the response to infection. It seems likely that the pro-inflammatory cytokines play an important role in the host response to T. annulata. Our findings suggest that the pro-inflammatory cytokines are suitable markers of inflammatory reactions in T. annulata-infected cattle.
11. Abdalla OA, EL-Boshy ME, Hamid FMA, and Ali NM (2013). ‘Clinicopathological studies of dietary supplementation of Saccharomyces cerevisiae in calves.’ Journal of American Science; 9.
http://www.jofamericanscience.org/journals/am-sci/am0909/039_20600am0909_398_406.pdf
(Laboratory Medicine Department)
ABSTRACT
Background and Objectives: The present study aimed to evaluate some selective immunological,
antioxidant and biochemical parameters as well as growth promoters effects of Saccharomyces cerevisiae supplementation as a feed additives in calves. Material and Methods: Thirty buffalo calves were divided into 3 groups. Group I: control one, Group II: treated orally with Saccharomyces cerevisiae (Sc) at a dose rate 30g once daily/calf for 12 weeks and groupIII: treated with 60g Sc once daily/calf for 12 weeks. Results: The treatment with Saccharomyces cerevisiae resulted in significant increase in the body weight, blood glucose level, total protein, albumin and A/G ratio, while AST, LDH activities, globulin concentration, uric acid and creatinine levels are insignificantly changed. The level of MDA is significantly decrease. Meanwhile catalase, GSH level, serum lysozyme, bactericidal activity and serum NO level are significantly increased Conclusions: we are concluded that
Saccharomyces cerevisiae treatment of the calves has desirable effects on the body weight, serum antioxidant enzymes and some immunological parameters. Also has no toxic effect on kidney or liver functions.
12. Abdalla OA, EL-Boshy ME, El-Khodary SA, Reisha EF and Gadalla HA (2013). ‘Selective Serum Oxidant, Antioxidant and Trace Elements Profile in Ossimi Sheep Affected with Pregnancy Toxemia.’ Life Science Journal; 10:2833-2837.
(Laboratory Medicine Department)
ABSTRACT
Pregnancy toxemia is a metabolic disorder affecting mainly sheep usually during last period of gestation specially those bearing twines and reflected by nervous signs in affected animals. A totally (265) examined sheep, about (8) cases was tentatively diagnosed as pregnancy toxemia according to the previous case history, present clinical signs and serum biochemical analysis. Our serum chemistry parameters revealed a significant elevation in creatine kinase (CK) activity, β-Hydroxybuteric acid (βHBA), creatinine and urea levels with a significant reduction in glucose value in pregnancy toxemia comparing to control pregnant. Oxidative stress and antioxidant biomarkers in serum from pregnancy toxemic sheep show a significant increase in hydrogen peroxide (H 2 O 2) level more than control pregnant ones. Also, a significant decrease of glutathione peroxidase (GPx) activity, total antioxidant capacity (TAC) and vitamin C (Vit C) in pregnancy toxemia group compared to control pregnant group. The trace elements study revealed a significant increase in the level of copper (Cu) and iron (Fe) while a marked decrease of zinc (Zn) value in pregnancy toxemia cases than control pregnant group. In conclusion, the oxidative stress markers and antioxidants with trace elements analysis could provide a great overview for their role in pathogenesis of pregnancy toxemia in Ossimi sheep.
13. El-Boshy ME, Abdalla OM, Risha A, and Moustafa F (2013). ‘Effect of Withania somnifera Extracts on Some Selective Biochemical, Hematological, and Immunological Parameters in Guinea Pigs Experimental Infected with E. coli.’ ISRN veterinary science; 2013:153427.
http://www.hindawi.com/journals/isrn/2013/153427/
(Laboratory Medicine Department)
ABSTRACT
Fifty 1-2-month-old Guinea pigs were divided into 5 equal groups, 10 each. Control (Gp1) did receive neither viable bacteria nor treatment. Each animal from the other groups (Gp2-5) was challenged with (1-2 × 10(8)) viable E. coli in 200 μ L normal saline (0.9%) through IP route. GP2 infected group was treated with 200 μ L saline IP and kept as positive control group. Gp3-4 are infected and treated with Withania somnifera (ethanol root extract) with doses 50 and 100 mg/kg. BW, respectively. Gp5 infected treated group was treated with cefoperazone antibiotic at dose 35 mg/Kg BW. The treatment by drug or the extracted medicinal plant was started 72 h after infection for 7 successive days. Serum and whole blood sample were collected from all groups 14 days after treatment to evaluate some hematological and biochemical changes as well as immunomodulatory cytokine tumor necrosis factor-alpha (TNF- α ). Oral treatment of the plant extract caused significant benefit results in infected Guinea pig appeared in the correction of some hematological and biochemical parameters also try to suppressed inflammatory cytokine response represent in TNF- α . It could be concluded that W. somnifera extract has potent antibacterial activity, and this appears in the correction with hematological, biochemical, and immunological results.
14. Al-Kushi AG, El-Boshy ME, ElSawy NA, Omar OSO, Header EA (2013). ‘Pathological Comparative Studies on Aqueous and Ethanolic Extracts of Zingiber officinale on Antioxidants and Hypolipidemic Effects in Rats.’ Life Science Journal; 10: 2393-2403.
(Laboratory Medicine and Clinical Nutrition Departments)
ABSTRACT
Objectives: This study was designed to evaluate the effect of Zingiber officinale extract on oxidative
stress and plasma lipid profile in rats through antioxidant enzymatic activities, some selective biochemical analysis and histopathological examination on liver, kidney and stomach in rats through pathological investigation by histpathological and ultra-structure lesions. Methods: Thirty male albino rats of body weight 150-200 gm, were divided randomly into 5 equal groups as follow: Group I; normal control, Groups; II & III; received aqueous extract of Zingiber officinale (200 & 400 mg/kg b wt.) respectively. Groups; IV & V received Zingiber officinale ethanol extract (200 & 400 mg/kg b wt.) respectively. The extracts were orally administered daily for 30 days. At the end of experiment period, tissues specimens were obtained from all groups and were fixed in 10% neutral buffered formalin for histopathological and ultra-structure examination. Results: GSH level revealed significant increase in all Zingiber officinalis treated groups, compared to the control. SOD showed significant increase and MDA decrease in group IV only compared to the control group. Serum Triglycerides level reveled significant decrease in Zingiber officinalis treated groups (II, IV, V) compared to the control. Serum total cholesterol and cholesterol -LDL levels showed significant decrease in all Zingiber officinalis treated groups compared to the control. Liver transaminase activities and urea serum level showed significant increase in higher dose of ethanol extract (Gp. V) compared to control group. The pathological lesions were observed only in higher dose of Zingiber officinale ethanol extract, mild vacuolar degeneration of hepatocytes. The renal lesions were observed by marked granularity of the cytoplasm renal tubules, also ultra-structures approved multiple cytoplasmic vacuoles with heterochromatic nucleus, moreover tunica intima of renal artery fused with tunica media. The gastric examination showing sloughing of gastric mucosa with leukocytes infiltration. Conclusion: Although Zingiber officinale extracts have been documented an effective in hypolipidemic effects and exerting antioxidant effect by enhance antioxidant activities and reduce oxidative stress. The present results conclude that, the Zingiber officinale aqueous extract more safe in compare with ethanol extract and more further studies are recommended to evaluate Zingiber officinale side effect regarding to the type of extracts, doses and duration administration.
15. El-Boshy M and Abdall O (2013). ‘Effect of lactoferrin on some selective immunological parameters in rats immunosuppressed by cyclophosphamide.’ Journal of Investigational Biochemistry; 2: 136-140.
http://www.scopemed.org/?mno=37078
(Laboratory Medicine Department)
ABSTRACT
Eighty male albino rats (350±10 g) 10 to 12 weeks old were conducted in our study. Rats
were randomly divided into four, equal groups. The groups treated as following: 1st control
group (Gp. A) was given intraperitoneal normal saline (1 mL). 2nd group (Gp. B) was given a
single intraperitoneal dose (250 mg/kg body weight) of Cyclophosphamide (CP) on the first
day of the experimental period. 3rd group (Gp. C) CP and lactoferrin (LF) treated group. 4th
group (Gp. D) administrated LF only (0.5%) in drinking water. Two separate blood samples
were collected from heart puncture at end of 1st and 3rd week post treatment for
hematological, biochemical and immunological studies. The leukogram of CP treatment
group showed severe leucopenia (lymphopenia, neutropenia as well as eozinopenia) as well
as decrease total protein and albumin blood level. Immunosuppressive effect of CP is
documented in our work by decrease gamma globulin and elevation tumor necrosis factor
Alfa (TNF ά). This study revealed that oral treatment with LF can partially reconstitute
humoral and cellular immune response in rats given a sub-lethal dose of CP.
16. El-Ashker M, Salama M, and El-Boshy M (2013). ‘Traumatic reticuloperitonitis in water Buffalo (Bubalus bubalis): clinical findings and the associated inflammatory response.’ Journal of Veterinary Medicine; 2013.
http://www.hindawi.com/journals/jvm/2013/808656/
(Laboratory Medicine Department)
ABSTRACT
The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful.
17. Elgazar AF, Rezq AA and Bukhari HM (2013). ‘Anti-Hyperglycemic Effect of Saffron Extract in Alloxan-Induced Diabetic Rats.’ European Journal of Biological Sciences; 5: 14-22.
http://www.idosi.org/ejbs/5(1)13/3.pdf
(Clinical Nutrition Department)
ABSTRACT
Saffron (Crocus sativus) from iridaceous genus is a stable grass and is considered as an important herb in medical, cosmetics and hygienic industries. The aim of present study was to investigate the effect of given oral administration of saffron water extract with different dosages on alloxan-induced diabetic rats. Thirty five male albino rats of Sprague-Dawley strain weighing 200 ± 5 g were divided into five groups of equal number and weight. Group I, normal control rats; group II, diabetic control rats; and groups III, IV and V, diabetic rats, given orally saffron extract by tube feeding at levels of 200, 400 and 600 mg/kg of body weight, respectively. Oral administration of saffron extract at the three different doses caused significant increase in body weight and serum insulin level in all treated diabetic groups, while significantly reduced blood glucose levels as well as the improvement in lipid profile and liver and kidney functions compared to the positive control group. Histological study showed that pancreas sections of rats from positive control group had hypertrophy and hyperplasia of β-cells of islets of langerhans associated with pyknosis of their nuclei. However, treated rats with 200 mg/kg b.wt of saffron extract had vaculations of acinar epithelial lining in pancreas. Slight hypertrophy of islets of langerhans was demonstrated in pancreas of treated rats with 400 mg/kg b.wt. Apparently normal histological structure of pancreas was found in treated group with 600 mg/kg b.wt. In conclusion administration of saffron extract reduced blood glucose level and the incidence of different complications as results of hyperglycemia. Saffron have an advantage due to the presence of associated bioactive compounds with antioxidant properties which may exert further health promoting effects.
18. AbdulJawad SA, Hasanein MA, Header EA (2013). ‘Influence of Aqueous Extract of Red Chillis Pepper as Curative for Gastric Ulcer in Albino Rats.’ Journal of Natural Sciences Research; 3(4).
http://www.iiste.org/Journals/index.php/JNSR/article/view/4904
(Clinical Nutrition Department)
ABSTRACT
The present study was carried out elucidate the effect of aqueous extracts of red chillis pepper (Capsicum frutescence) at three doses on the length of gastric ulcer induced by aspirin in rats. Thirty adult male albino rats (Sprague Dawley Strain) weight 175±5g were used and divided into 5 groups, each of 6 rats. The first group was used as a control negative (-ve) and fed on the basal ration only, other groups had given aspirin orally (200 mg/kg B.Wt.), one of these groups left as control +ve (ulcerated rats) and other groups administrated with aqueous extract of red chillis pepper (RCP) at doses of 250, 500 and 750 mg/kg B.Wt. for seven days. The length of gastric ulcer, volume of gastric juice, pH value and histopathological changes of gastric were examined. The results revealed that oral administration of RCP extract at 250, 500 and 750mg/kg B.Wt. reduced the length of gastric ulcer. On the other hand, all extracts increased pH value of gastric juice compared to control (-ve) group, meanwhile the volume of gastric juice decreased by plant extracts specially for rats feed on RCP at dose 750 mg\Kg B.Wt. Oral administration of water plant extracts decreased histopathological changes in the stomach layers and mucosa. The present study suggests that, Capsicum frutescence could be used for healing acute gastric ulcer disease and implemented for gastric ulcer patients
19. Kensara OA (2013). ‘Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats.’ International Journal of Nutrition and Metabolism; 5: 61-68.
http://www.academicjournals.org/journal/IJNAM/article-abstract/62C4CFA5002
(Clinical Nutrition Department)
ABSTRACT
Recent studies have suggested a potential direct role of mild hyperuricemia in development of chronic kidney disease independent of urate crystal formation. The present study was designed to investigate the possible anti-hyperuricemic and renoprotective effect of vitamin C, as a natural antioxidant with uricosuric property on a rat model with chronic mild hyperuricemia-induced nephropathy. A model of mild hyperuricemia was induced in male Wistar rats with an uricase inhibitor, oxonic acid (OA) (750 mg/kg per day for 4 weeks by gastric gavage). Rats were divided into four groups: (1) control; (2) OA only; (3) OA + vitamin C (200 mg/kg for 4 weeks by gastric gavage); and (4) vitamin C only. At the end of the study, rats were sacrificed under diethyl ether general anesthesia and serum levels of uric acid, creatinine and blood nitrogen urea (BUN) as well as glutathione (GSH) levels and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) were measured as indices of oxidative stress and anti-oxidative status in kidney tissues. Also, histopathological examination of isolated kidney tissues was performed. The administration of OA resulted in 2.4 fold increase in serum uric acid levels, and was associated with development of kidney damage characterized by a significant increase in serum levels of creatinine and BUN, and significant decreases in renal GSH levels and activities of SOD, GPx and GST.By contrast, simultaneous administration of vitamin C significantly ameliorated all these biochemical changes induced by OA. The histopathological findings supported these biochemical observations, whereby vitamin C supplementation remarkably reduced OA-induced tubulointerstitial damage and cellular infiltration in rat kidneys. These results indicate that vitamin C therapy significantly attenuated the biochemical indices, histopathological findings and oxidative stress parameters of OA-induced hyperuricemia and nephrotoxicity in rats. This may provide insight into the possible potential renoprotective effect of vitamin C supplementation against hyperuricemia nephropathy.
20. Kensara OA, ElSawy NA, El-Shemi AG, and Header EA (2013). ‘Thymus vulgaris supplementation attenuates blood pressure and aorta damage in hypertensive rats.’ Journal of Medicinal Plants Research; 7: 669-676.
(Clinical Nutrition & Laboratory Medicine Departments)
ABSTRACT
In the present study, we investigated the possible antihypertensive effect of Thymus vulgaris (T. vulgaris) and its possible protective role against hypertension-induced aorta damage in hypertensive rats. Hypertension was induced by ligation of left renal artery, and T. vulgaris aqueous extract was administered (100 mg/kg/day, orally) for 8 consecutive weeks. Systolic blood pressure, body weight, and the serum concentrations of creatinine and cholesterol were measured at the beginning and at the end of the study. Thoracic aorta was isolated at the end of the study for both light and electron microscopic examinations. The antihypertensive effect of T. vulgaris was clearly observed here, as well its significant reducing effect on hypertension-induced increases in serum levels of creatinine and cholesterol. The light microscopic findings indicated that the surface endothelium of thoracic aorta of hypertensive-untreated rats was coarse, wrinkled and protuberant, and its lumen adsorbed more debris and red blood cells; however, these phenomena were almost disappeared when these animals were treated with T. vulgaris. Similarly, the electron microscopic examinations showed a remarkable increase in aortic extracellular matrix with dispersion of their cell nuclei in hypertensive-untreated rats but not in rats treated with T. vulgaris. Therapy with T. vulgaris also reduced hypertension-induced aortic smooth muscle cell mass hypertrophy and normalized both aortic lumen diameter and media thickness. In conclusion, our results indicate that hypertension induced in rats was associated with injury of aortic tissue that may accelerate the arterial dysfunction in uncontrolled hypertensive conditions. More importantly, supplementation with T. vulgaris as herbal remedy has shown remarkable antihypertensive effect and marked improvement on hypertension-related biochemical changes and aortic vascular damage in rats.
21. Khan MA, Ashshi A, and Mahomed M (2012). ‘Drug resistance patterns of acinetobacter baumannii in Makkah, Saudi Arabia.’ Pakistan Journal of Medical Research; 51:127.
https://inis.iaea.org/search/search.aspx?orig_q=RN:44054708
(Laboratory Medicine Department)
ABSTRACT
Background: Acinetobacter baumannii causes infections of respiratory, urinary tract, blood stream and surgical sites. Its clinical significance has increased due to its rapidly developing resistance to major groups of antibiotics used for its treatment. There is limited data available on antimicrobial susceptibility of A.baumannii from Saudi Arabia. Objectives: To determine the patterns of drug resistance of Acinetobacter baumannii and predisposing factors for its acquisition. Subjects and Methods: In this descriptive study, 72 hospitalized patients infected with A.baumannii were studied. The clinical and demographic data of the patients were collected using a predesigned questionnaire. Isolation and identification of A.baumannii from all clinical specimens were done using standard microbiological methods. Antibiotic susceptibility testing was performed by disk diffusion method recommended by Clinical Laboratory Standards Institute. Results: Majority of the isolates (61.1%) were from respiratory tract infections. A.baumannii isolates showed high drug resistance to piperacillin (93.1%), aztreonam (80.5%), ticarcillin, ampicillin, and tetracycline (76.4%, each) and cefotaxime (75%). Only amikacin showed low rate of resistance compared to other antibiotics (40.3%). About 36% patients had some underlying diseases with diabetes mellitus (11%) being the predominant underlying disease. Conclusions: High antimicrobial resistance to commonly used antibiotics was seen against A.baumannii isolates. Only amikacin was most effective against it.
22. Johargy AK, Mahomed MF, Khan MM, and Kabrah S (2013). ‘Anti-hepatitis E virus seropositivity in a group of male blood donors in Makkah, Saudi Arabia.’ Journal of Pakistan Medical Association; 63: 185-189.
http://jpma.org.pk/full_article_text.php?article_id=3993
(Laboratory Medicine Department)
ABSTRACT
Objectives: To determine the seropositivity of Immunoglobulin-G and Immunoglobulin-M to hepatitis E virus in male blood donors in Makkah, Saudi Arabia. Methods: The study was carried out from March to August 2009, in which 900 blood samples were collected from 4 different hospital blood banks in Makkah City: AL-Noor Hospital, Central Blood Bank, Maternity and Children Hospital, and Herra Hospital. All the samples were tested for Immunoglobulin-G and Immunoglobulin-M antibodies specific to hepatitis E virus using the enzyme-linked immunosorbent assay test. Results: Hepatitis E virus-specific Immunoglobulin-G antibodies were detected in 168/900 (18.7%), and IgM in 39/900 (4.3%) samples. Prevalence of the former was found to be higher in non-Saudi donors. In addition, its prevalence increased with age. Moreover, its prevalence was found to be higher in uneducated donors and in donors who drank well-water. Conclusion: Exposure to hepatitis E virus among blood donors in Makkah City was high in comparison to the neighbouring areas in the region. Further studies are warranted to determine the true seroprevalence of the virus in the society at large.
23. Saleh HA and Khereldeen MM (2013). ‘Physicians’ Perception towards Patients’ Rights in Two Governmental Hospitals in Mecca, KSA.’ Int. J. Pure Appl. Sci. Technol; 17: 37-47.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.379.7&rep=rep1&type=pdf
(Health Management Department)
ABSTRACT
The Saudi society has its own unique values, morals, and ethics which are based on the religion of Islam. The social system in Saudi Arabia is very supportive of patients and their families, and in most hospitals patients have access to free healthcare services. However, there has been little discussion on how patients ’ rights are practiced in such healthcare systems. In this research paper the perception of physicians concerning patients ’ rights and their fulfilment in two governmental hospitals in Mecca, Saudi Arabia is compared, using a self-administered questionnaire which examined the physicians ’ knowledge, attitude and perception towards these rights.
24. Shaikh-Omar OA, Header EA and Bukhari HM (2013). ‘Digestive Discomforts and Effect of Ingested Food in People Performing Hajj.’ Journal of Basic & Applied Sciences; 9: 341-347.
http://www.lifescienceglobal.com/pms/index.php/jbas/article/view/1116
(Clinical Nutrition Department)
ABSTRACT
A variety of health issues have been investigated in the Hajj pilgrims (HPs) in KSA. However, it is still obscure to decide which meals contribute to health related issues specifically gastrointestinal disorders and allergies. Hence present study was planned to compare the effect of using different meals on GIT disorders and allergies.
The data was categorized into two groups. Group 1 of age matched HPs comprised a Functional Digestive System Questionnaire (FDSQ) to study digestive disorders. Group 2 HPs were compared for those received Toafa Corporation Meals (TCM) and those did not received Toafa Corporation Meals (NTCM).
The FDSQ showed some of the gastrointestinal symptoms in male and female Hajj pilgrims differing significantly (p<0.05). The efficacy of the treatment was evaluated by subject recovery scoring (SRS). The 61.7% HPs taking TCM revealed various discomforts and food allergy and the 50.6% HPs using NTCM did not suffer from any disorders. However, the pilgrims taking the meals provided by the Toafa corporation but not showing allergy were more significant in number (p=0.0002).
The existing study presents a new method (construction of a FDSQ) for comparing gastrointestinal and other disorders. The present study recommends that Toafa Corporation should provide suitable meals for the pilgrims to meet their actual needs during Hajj and health situation.
25. Elkamel AA and Mohamed AM (2012). ‘Differential Identification of Flavobacterium Species by Sequence Analysis of Genus-Specific Hypervariable 16S-23S rDNA Intergenic Spacer Target.’ World Journal of Fish and Marine Sciences; 4: 597-603.
http://www.idosi.org/wjfms/wjfms4(6)12/7.pdf
(Laboratory Medicine Department)
ABSTRACT
The aim of the current study was to develop a molecular system for differential identification of various fish diseases caused by Flavobacterium species. The system uses the hypervariability of the 16S-23S rDNA Intergenic spacer region (ISR) to develop PCR-based sequence analysis assay. For this purpose, eight different 16S-23S rDNA ISR sequences of six different Flavobacterium species were aligned and compared to detecta hypervariable region with conserved flanking sequences as a target for amplification. The conserved flanking regions were used to design primers that target the selected hypervariable ISR sequence from all Flavobacterium species. American Type Collection of Cultures and other reference strains were used to assess the precision and specificity of the system. The results revealed the ability of the described molecular assay to accurately identify all the ATCC and reference Flavobacterium species to the strain level. In addition, two clinical isolates that were conventionally identified in the current study as Flavobacterium psychrophilum and Flavobacterium columnare were re-identified, using the molecular assay as F. columnare and Flavobacterium johnsoniae, respectively. The currently described ISR sequence analysis-based differential identification assay provides rapid and accurate identification of the different diseases causing Flavobacterium species and represent a useful tool for successful epidemiological studies and management of Flavobacterium species-caused fish diseases.
26. Mohamed AM, Abou El Ella GA, and Hayder IA (2013). ‘Antioxidant effect of bee pollen on immune status of hyperglycemic rats.’ Assiut Vet Med J; 59: 107-116.
http://www.aun.edu.eg/journal_files/119_J_2533.pdf
(Laboratory Medicine Department)
ABSTRACT
The aim of the current study was to evaluate the ability of bee pollen (BP), a known antioxidant-rich food supplement, to prevent or minimize the immune insufficiency complications associated with diabetes mellitus (DM) in rat model. A total of 32 adult male Wistar Albino rats divided into 4 groups (8 rats each) were used in the current study. One group were fed basal diet only for 60 successive days and used as non- diabetic control group (G I), while the other groups were used for induction of DM model after injection with streptozotocin (STZ) 65 mg/kg. One of the diabetic rat groups received only basal diet without BP supplementation for the same period and used as diabetic control group (G II). The other diabetic groups were subdeivided into 2 subgroups, which received basal diet supplemented with BP at a concentration of 1% and 2% for 60 successive days and assigned as G III and G IV, respectively. The obtained results revealed that at the end of the experiment there are still significant increases (P≤0.001) in glucose levels in groups II, III and IV in comparison to G I. However, non-significant changes were recorded in glucose levels G III & IV as compared G II. With regard to oxidative stress, significant reduction (P ≤ 0.01) were recorded in the levels of evaluated antioxidant parameters (SOD, CAT and GSH-PX) in G II as compared to G I. However, significant increases (P ≤ 0.01) and (P ≤ 0.001) were recorded in the levels of antioxidant parameters in G III and G IV, respectively as compared to G II of hyperglycemic rats. With regards to immune status, significant reduction of CIC (P ≤ 0.01), phagocytic activity of neutrophils (P ≤ 0.01) and IFN-gamma (P ≤ 0.001) were recorded G II as compared to G I. On the other hand, significant increase were recorded in the levels of IgG (P ≤ 0.01) and IgM (P ≤ 0.001) in G II as compared to G I. However, significant improvement of CIC (P ≤ 0.05 and P ≤ 0.01), Phagocytic activity (P ≤ 0.05 and P ≤ 0.01), IgM (P ≤ 0.05 and P ≤ 0.01), IgG (P ≤ 0.05 and P ≤ 0.01) and IFN-gamma (P ≤ 0.01 and P ≤ 0.001) were recorded among G III and G IV, respectively as compared to G II. In conclusion, an association between oxidative stress and immune insufficiency were recorded in the present study in diabetic rats Moreover, an obvious improvement of immune status parameters were recorded after supplementation of diabetic rats with bee pollen as an antioxidant-rich food supplement.
27. Zaglool D, Mohamed A, Khodari Y, and Farooq M (2013). ‘Crypto-Giardia antigen rapid test versus conventional modified Ziehl-Neelsen acid fast staining method for diagnosis of cryptosporidiosis.’ Asian Pacific journal of tropical medicine; 6: 212-215.
http://www.sciencedirect.com/science/article/pii/S1995764513600255
(Laboratory Medicine Department)
ABSTRACT
Objective: To evaluate the validity of Crypto-Giardia antigen rapid test (CA-RT) in comparison with the conventional modified Ziehl-Neelsen acid fast (MZN-AF) staining method for the diagnosis of cryptosporidiosis. Methods: Fifteen preserved stool samples from previously confirmed infections were used as positive controls and 40 stool samples from healthy people were used as negative control. A total of 85 stool samples were collected from suspected patients with cryptosporidiosis over 6 months during the period from January till June, 2011. The study was conducted in the department of parasitology, central laboratory, Alnoor Specialist Hospital, Makkah, Saudi Arabia. All samples were subjected to CA-RT and conventional MZN-AF staining method. Validation parameters including sensitivity (SN), specificity (SP), accuracy index (AI), positive predictive value (PPV), and negative predictive value (NPV) were evaluated for both tests. Results: Out of 15 positive controls, CA-RT detected 13 (86.7%) while MZN-AF detected 11(73.3%) positive cases. However, CA-RT detected no positive case in 40 normal controls but MZN-AF detected 2(5%) as positive cases. Based on the results, the SN, SP, AI, PPV and NPV were high in CA-RT than MZN-AF staining method, ie., 86.7%vs. 73.3%, 100%vs. 95%, 96.4%vs. 89.1%, 100%vs. 84.6% and 95.2%vs. 90.5%, respectively. Out of a total of 85 suspected specimens, CA-RT detected 7(8.2%) but MZN-AF detected 6(7.1%) cases as positive. Conclusions: CA-RT immunoassay is more valid and reliable than MZN-AF staining method.
28. El-Ashmony SMA, Morsi HK, and Abdelhafez AM (2012). ‘Effect of Zinc Supplementation on Glycemic Control, Lipid Profile, and Renal Functions in Patients with Type II Diabetes: A Single Blinded, Randomized, Placebo-Controlled, Trial.’ Journal of Biology, Agriculture and Healthcare; 2: 33-41.
http://www.iiste.org/Journals/index.php/JBAH/article/view/2260
(Laboratory Medicine Department)
ABSTRACT
This study was conducted to evaluate the effect of zinc supplementation on glycemic control, lipid profile, and kidney functions in patients with type 2 diabetes mellitus attending the diabetic centre of Alnoor, Specialized Hospital, in Makkah, Saudi Arabia .A single blinded, randomized, placebo-controlled, trial was conducted. Patients (n=60) were randomly allocated into two groups: zinc group, and placebo group, treatment was given for 8 weeks. Fasting blood glucose (FBG), glycated hemoglobin (HbA1c%), kidney functions and lipid profile were assessed at baseline and after 8 weeks. Results showed that FBG, HbA1c%, lipid profile, and kidney functions were significantly reduced in zinc group after 8 weeks compared to their levels before supplementation. Moreover, FBG, cholesterol, LDL, and LDL/ HDL ratio were significantly decreased, while HDL was significantly increased in zinc group compared to those in placebo group. Zinc may have supplementary benefits in the routine management of adult DM.
29. Zalata AA, Morsy HK, Badawy AE-N, Elhanbly S, and Mostafa T (2012). ‘ACE gene insertion/deletion polymorphism seminal associations in infertile men.’ The Journal of urology; 187: 1776-1780.
http://www.jurology.com/article/S0022-5347(11)06023-X/abstract
(Laboratory Medicine Department)
ABSTRACT
Purpose: We assessed seminal associations of the ACE gene insertion/deletion polymorphism in infertile men. Materials and Methods: A total of 405 men were investigated, divided into healthy fertile men, and those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. They underwent semen analysis, and assessment of sperm acrosin activity, hypo-osmotic swelling, seminal 8-iso-prostaglandin-F(2α), total antioxidant capacity, α-glucosidase and ACE gene polymorphisms. Results: The ACE insertion/insertion genotype was noted in 182 men, including 76.5% of healthy fertile men, and 47.4%, 39.8% and 17.6% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE insertion/deletion genotype was noted in 133 men, including 13.7% of healthy fertile men, and 42.3%, 27.5% and 47.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE deletion/deletion genotype was identified in 90 men, including 9.8% of healthy fertile men, 10.3%, 32.70% and 35.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. Men with the ACE deletion/deletion and insertion/deletion genotypes showed a significant decrease in sperm count, motility, linear velocity and normal forms, acrosin activity index, hypo-osmotic swelling test and seminal α-glucosidase, and significantly increased seminal 8-iso-prostaglandin-F(2α) than those with the ACE insertion/insertion genotype. Conclusions: ACE gene deletion polymorphism is associated with abnormal seminal variables, such that carriers of the ACE deletion/deletion genotype have higher seminal oxidative stress.
30. Shalaby and Morsy HK (2013). ‘Effect of pregabalin on erectile function and penile NOS expression in rats with streptozotocin-induced diabetes.’ Exp Clin Endocrinol Diabetes; 121: 230-3.
https://www.thieme-connect.com/DOI/DOI?10.1055/s-0033-1341428
(Laboratory Medicine Department)
ABSTRACT
Objectives: To investigate the effect of pregabalin on erectile function and on the penile expression of nitric oxide synthase (NOS) isoforms in diabetic rat models. Methods: Male Sprague-Dawley rats were injected with streptozotocin to induce diabetes mellitus. The rats with blood glucose levels above 300 mg/dL were selected for the study. Those were randomly divided into 2 groups (N=10 per group): i) EDDM (erectile dysfunction diabetes mellitus) group fed with saline and ii) EDDM+Pregabalin treated group receiving pregabalin (10 mg/k/day) by intragastric administration. 10 animals served as a control group and received no streptozotocin. 4 weeks later, the erectile function of the rats was assessed by recording frequency of erection after subcutaneous apomorphine (80 ug/kg) injection. Superoxide anion generation and the expression of mRNA of nNOS and eNOS were evaluated in corpora cavernosum tissue. Results: Penile erection, the expression of both nNOS, eNOS and superoxide generation were significantly decreased in pregabalin treated group compared to control group. Conclusion: Treatment with pregabalin for 4 weeks decreases superoxide production but cannot improve erectile dysfunction in diabetic rats probably by inhibiting Ca(2)(+) channel-mediated NOS activation.
31. Osfor M, Kensarah OA, Azzeh FS, Elmadbouly M and El-Qutry M (2013). ‘Biochemical changes in lipid and carbohydrate metabolism in obese diabetic male albino rats.’ International Journal of Nutrition and Food Sciences; 2: 24-30.
http://www.eurofedlipid.org/meetings/archive/cracow/5877/5877_0069.pdf
(Clinical Nutrition Department)
ABSTRACT
The effect of high fat diet on lipid and carbohydrate metabolic changes was studied in mature male albino rats. The animals were rendered obese by feeding a high fat diet for 60 days. Half of obese rats were become diabetic by injecting with a reduced dose of alloxan; to produce a non-insulin dependent diabetes mellitus state. These obese models of non-diabetic and diabetic rats were compared with normal male rats kept on a basal diet for 60 days. The fat fed rats beside gaining weight showed a significant rise in their hepatic lipid contents and observed an insulin resistant state. Lipid profile of the omental adipose tissue exhibited a significantly higher total lipid content, but lower total cholesterol, triglycerides and phospholipids contents in both obese (non-diabetic and diabetic) as compared with the normal rats. On the other hand, the brown adipose tissue showed a reduced fat catabolism with a significant rise in its phospholipid contents. Correlation between plasma atherogenic index and plasma fatty acids pattern showed that in the obese diabetic rats atherogenic index was positively correlated with total polyunsaturated fatty acids, while significantly negative correlation with total saturated fatty acids. This work showed that high fat substrates have been got remarkable impacts on rats body weight and metabolism.
32. Osfor MMH, Hegazy A, Abd El-moaty M, Elmadbouly MA, Afify AMR and AElbahnasawy ASM (2013). ‘Hypo-cholesterolemic and hypoglycemic effects of orange albedo powder (citrus aurantium I) on male albino Rats.’ International Journal of Nutrition and Food Sciences; 2: 70-76.
http://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130202.17.pdf
(Clinical Nutrition Department)
ABSTRACT
The present study was conducted to evaluates the hypocholesterolemic and hypoglycemic effects of dietary Orange Albedo Powder (Citrus Aurantium L.) in the hypercholesterolemic albino rats, also to examine various oxidative stress-associated with some biochemical parameters. Thirty two male albino rats weighing 110 + 10 g were divided into four groups, the first group received the basal diet only and served as (negative control), the second group received the hypercholesterolemic diet and served as (positive control), the other two groups received hypercholesterolemic diet supplemented with 10%, 20% orange albedo powder respectively (OrAP) for six weeks spontanously. The obtained results revealed that the groups of rats which received diets supplemented with 10% and 20% OrAP were significantly decrease total lipid, total cholesterol, triglycerides, low density lipoprotein cholesterol, liver enzymes (alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase), when compared with positive control, also kidney functions were significantly improved. Body weight gain and food intake were significantly decreased when compared with positive control. The results indicate that the orange albedo is a good source of natural fiber. It could use in obese people for body loss, also serves to improve blood picture and to reduce the blood glucose level in hypercholesterolemic rats.
33. Osfor MMH, Elmadbouly MA, Elsoadaa1 SS, Metair AH and Hussain AAA (2013). ‘Relation between Hypercholesterolemia and Insulin like growth factor-1 in Elderly Women suffer from Hypothyroidism.’ Journal of Natural Sciences Research; 3: 160-164.
http://www.iiste.org/Journals/index.php/JNSR/article/view/5315
(Clinical Nutrition Department)
ABSTRACT
The present study included 40 female subjects. They were classified into two groups: group I included 20 females diagnosed as primary hypothyroidism with age ranged between 45 – 65 years and group II included 20 ages matched normal female volunteers and served as control group. Insulin like growth factor -1, (IGF-1) cholesterol, triglycerides, Thyroid stimulating hormone, thyroxine-3 (T3) and thyroxine-4 (T4) hormones were measured in both groups. The obtained results revealed that, there was a significant decrease in IGF-1 level in group I, when compared to control group (P < 0.01). Correlation studies showed that IGF-1 is strongly positively correlated with T3 (R=0.81, P < 0.01) and moderately positively correlated with T4 (R= 0.58, P < 0.05). Meanwhile, it was moderately negatively correlated with cholesterol (R= 0.6, P < 0.05). The results indicate that IGF-1 deficiency in elder women suffering from ?hypothyroidism may have a direct relation in the pathogenesis of hypercholesterolemia.
34. Osfor MMH, El-Ashi A, El-Madbouly M, Zaki MS and Negm D (2013). ‘A prospective study of Green Tea and panax Ginseng against mutagens & carcinogens produced during Thermolyzed Meat and Fish.’ Life Science Journal; 10: 1754-65.
http://www.lifesciencesite.com/lsj/life1001/255_16335life1001_1754_1765.pdf
(Clinical Nutrition Department)
ABSTRACT
Effect of thermolyzed (ultra-heated) Meat and fish on the nutritional, biochemical, histological, and hematological parameters as well as aberrations and sperm abnormalities was investigated in male albino rats. Animals were divided into two main groups: Meat group and Fish group. Each group was divides into six subgroups: G-I was fed the basal diet containing more than 12% dry frozen meat, G-II & G-III were fed the basal diet of G-I plus 5% of Green Tea and Panax Ginseng respectively. G-IV was fed the basal diet containing more than 12% Thermolyzed Meat, while, G-V & G-VI were fed the basal diet of G-IV plus 5% of Green Tea and Panax ginseng respectively. The same classification was done for the fish groups. It was found that the terminal body weight was increased non-significantly in all rat groups until the fifth week, and then decreased non-significantly until the end of the experimental periods (8 weeks). No effect on serum cholesterol, triglycerides, total lipids and glucose levels was found. On the other hand, significant values of both hepatic and renal function parameters were obtained among ultra-heated meat and fish treated animals. Numerous histological alterations, particularly in the liver, were also observed. Moreover, anemia, leukocytosis, neutophilia, lymphocytosis and monocytosis were seen. Furthermore, chromosomes and sperms were adversely affected in such animals fed diet contained ultra-heated meat and fish. The protective role of green tea and panax ginseng were observed in all biochemical, histological, and hematological parameters. In addition, improvement in chromosomal aberration and sperm abnormalities was also noticed.
35. Saleh HA and Kheeeldeen MM (2013). ‘Physicians’ perception towards Patients’ Rights in tow Governmental Hospitals in Mecca, KSA.’ International Journal of Pure Applied Sciences and Technology; 17: 37-47.
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.379.7&rep=rep1&type=pdf
(Health Management Department)
ABSTRACT
The Saudi society has its own unique values, morals, and ethics which are based on the religion of Islam. The social system in Saudi Arabia is very supportive of patients and their families, and in most hospitals patients have access to free healthcare services. However, there has been little discussion on how patients' rights are practiced in such healthcare systems. In this research paper the perception of physicians concerning patients' rights and their fulfilment in two governmental hospitals in Mecca, Saudi Arabia is compared, using a self-administered questionnaire which examined the physicians' knowledge, attitude and perception towards these rights.
