جامعة أم القرى
1. Abdelghany HA and Header EA, Seham A Khedr and Bukhari HM (2012). Hepatorenal protective effect of yoghurt pillared with propolis on normal and diabetic rats. J home economics, Minufiya University. (Non-ISI).
2. Bukhari SZ, Hussain WM, Banjar AA, Fatani MI, Karima TM, and Ashshi AM (2012) Application of ventilator care bundle and its impact on ventilator associated pneumonia incidence rate in the adult intensive care unit. Saudi medical journal; 33: 278-283. (ISI; IF = 0.62; Cited 4).
3. Ashshi AM (2012) Detection of occult hepatitis B virus in anti-HBc positive/anti-HBs positive blood donors in Saudi Arabia. Res J Med Sci; 6: 61-65. (Non-ISI; Cited 1)
4. Ashshi AM (2012) Is Rotavirus Infection Still Responsible for Acute Gastroenteritis and Severe Diarrhea among Children in Holy Makkah? Research Journal of Medical Sciences; 6: 170-174. (Non-ISI)
5. Azzeh FS (2012). Camel Milk as Functional Food: Review Paper (In Arabic). Arab Journal of Food and Nutrition; 29: 94-111. (Non-ISI)
6. Azzeh FS (2012). Relationship between Vitamin D and Rheumatoid Arthritis Disease: Review Paper. Pakistan Journal of Nutrition, 11(3): 293-300. (ISI; NO IF)
7. Bukhari HM, Abdelghany HA, Nada IS and Header EA (2012). Effect of yoghurt pillared with propolis on hyperglycemic rats. Egyp J Hospit Med, 49:691-704. (Non-ISI)
8. Bukhari HM, Nada IS and Header EA (2012). Effect of obesity and dietary factors on bone mineral density levels among female students in Umm Al-Qura University. Egyp J Hospit. Med; 49:678-690. (Non-ISI)
9. Bukhar HM, Bakri EH, Ali H, and Header EA (2012). Hepatorenal Protective Effect Of Yoghurt Pillared With Propolis On Normal And Hyperglycemic Rats. Home Econ. J.; 22. (Non-ISI).
10. Ebid AA, El-Shamy SM, Thabet AA (2012). Effect of Posterior Tibial Nerve Stimulation and Trospium Hydrochloride in Treatment of Overactive Bladder Syndrome: a randomized controlled study. Indian Journal of Physiotherapy and Occupational Therapy; 6: 36-41. (Non-ISI)
11. Elmadbouly MA and Abd Elhafez AM (2012). Assessment of Nutritional Status of Hospitalized Elderly Patients in Makkah Governorate. Pakistan Journal of Nutrition, 11: 984-990. (ISI; Cited 2).
12. Kensarah OA and Azzeh FS. (2012). Vitamin D Status of Healthy School Children from Western Saudi Arabia. Pakistan Journal of Nutrition, 11: 288-292. (ISI, NO IF; Cited 3).
13. Kensarah OA and Azzeh FS (2012). Implementing High Vitamin C Treatments to Decrease Blood Uric Acid Levels in Hyperuricemic Saudi Patients. Journal of American Science; 8: 462-467. (Non-ISI).
14. Kensara OA, ElSawy NA, and Header EA (2012) Aqueous Extract of Thymus Vulgaris-induced Prevention of Kidney Damage in Hypertensive Adult Male Albino Rat: Biochemical and Ultrastructural Study. Pakistan Journal of Nutrition; 11: 367-374. (ISI)
15. Bukhar HM, ElSawy NA, and Header EA (2012) Biological effect of high energy drink on normal and hyperglycemic rats. Pakistan Journal of Nutrition; 11: 301-309. (ISI; Cited 1)
16. El-Shemi AG and Faidah H (2011). Synergy of daptomycin with fusidin against invasive systemic infection and septic arthritis induced by type VI group B streptococci in mice. Afr. J. Pharm. Pharmacol. 5:1125-1131 (Non-ISI)
17. El-Shemi AG, Basalamah MA, Kensara OA, Ashshi AM (2011). Interleukin-22 therapy attenuates the development of acute pancreatitis in rats. J. Clin. Med. Res. 3:82-88 (Non-ISI; cited 4)
18. Sheweita SA, Yousef MI, Baghdadi HH, Elshemy AG (2012). Changes of drug metabolizing enzymes in the liver of male sheep exposed to either cypermethrin or dimethoate. Drug Metab Lett. 6:2-6 (ISI; cited 3)
19. Thabet AA, Helal OF, El-Shamy SM (2012). Efficacy of Low-Intensity Pulsed Ultrasound on Bone Mineral Density in Osteoporotic Postmenopausal Women. Indian Journal of Physiotherapy & Occupational Therapy; 6: 238-242. (Non-ISI)
20. El-Shamy SM, Abdelaal AAM and Helal OF (2012). Ventilatory response to aerobic exercise in obese children. Bioscience Research 9(1): 35-40. (Non-ISI)
21. Elsayed GM, Ismail MM, and Moneer MM (2011) Expression of P-glycoprotein, Cyclin D1 and Ki-67 in Acute Lymphoblastic Leukemia: Relation with Induction Chemotherapy and Overall Survival. Indian Journal of Hematology and Blood Transfusion; 27: 157-163. (ISI; Cited 1)
22. Mohamed A, Abou Elella G, Nasr E, and Soliman Y (2012) Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals. J Mycobac Dis S; 2: 2161-1068. (Non-ISI)
23. Mohamed A, Abdel-Rady A, Ahmed L, and El-Hosary A (2012) Evaluation of indirect TaSP enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in Egypt. Veterinary parasitology; 186: 486-489. (ISI; Cited 2).
24. Ali MME, El-Megeid AAA and Mostafa RAA (2012). Effect of some levels from ginseng, barley and carob on lipid profile and kidney functions of rats fed on high fructose diet. Journal of American science; 8: 152-162. (Non-ISI).
25. Refaat B and Ledger W (2011) The expression of activins, their type II receptors and follistatin in human Fallopian tube during the menstrual cycle and in pseudo-pregnancy. Human reproduction; 26: 3346-3354. (ISI; Cited 5)
26. Refaat B, Simpson H, Britton E, Biswas J, Wells M, Aplin JD, and Ledger W (2012) Why does the fallopian tube fail in ectopic pregnancy? The role of activins, inducible nitric oxide synthase, and MUC1 in ectopic implantation. Fertility and sterility; 97: 1115-1123. (ISI; Cited 9)
ملخص الأبحاث المنشورة 2011-2012
1. Abdelghany HA and Header EA, Seham A Khedr and Bukhari HM (2012). ‘Hepatorenal protective effect of yoghurt pillared with propolis on normal and diabetic rats.’ J home economics, Minufiya University.
(Laboratory Medicine & Clinical Nutrition Departments)
ABSTRACT
Many preventive and therapeutic effects of yoghurt have been mentioned. propolis is a resinous material collected by honey bees and it has antimicrobial, anti-inflammatory properties. Liver and kidney are affected by diabetes mellitus which may be induced by streptozotocin. This work aimed to study the hepatorenal protective effect of yoghurt pillared with propolis on normal and hyperglycemia rats. Thirty-six rats (Male Sprague-Dawley) of 12 wk old, weighing 140-180g were used in this study and fed basal diet for 1 week for adaptation. The rats were divided into two groups (18 normal and 18 diabetic rats). Diabetes was induced in one group (18 rats) via intra-peritoneal injection of by 65 mg/kg of streptozotocin (STZ). One normal and one diabetic group fed on basal diet while other groups of rats were fed on basal diet + 10% of yoghurt pillared with 10% and 25% propolis. At the end of experiment period 6 weeks, blood samples were collected and liver and kidney of each rat was removed rapidly and weighted separately. Normal and streptozotocin-induced diabetic rats were used and examined for serum level of liver enzymes and serum level of urea and creatinine. Histopathological examination was done for specimens of the liver and kidney of both normal and diabetic rats. Streptozotocin-induced diabetic rats showed increased levels of liver enzymes together with derangements of hepatic cells and sinusoids. There were increased levels of serum urea and creatinine with disturbances of glomerular cells and fragmentation of the renal tubules. Yoghurt pillared with propolis reverse these effects with lowering of the serum levels of liver enzymes and urea and creatinine and reversal of the damage of the liver and kidney. The study also showed that yoghurt with propolis had a hypoglycemic effect.
2. Bukhari SZ, Hussain WM, Banjar AA, Fatani MI, Karima TM, and Ashshi AM (2012). ‘Application of ventilator care bundle and its impact on ventilator associated pneumonia incidence rate in the adult intensive care unit.’ Saudi medical journal; 33: 278-283.
http://www.ncbi.nlm.nih.gov/pubmed/22426908
(Laboratory Medicine Department)
ABSTRACT
Objective: To reduce ventilator associated pneumonia (VAP) incidence rate, lessen the cost of care, and correlate VAP bundles compliance with VAP incidence rate. Methods: This study was a prospective longitudinal study conducted on adult intensive care unit (ICU) patients at Hera General Hospital, Makkah, Kingdom of Saudi Arabia from January to December 2010. The following Institute for Healthcare Improvement VAP prevention bundle was applied: head-of-bed elevation; daily sedation-vacation along with a readiness-to-wean assessment; peptic ulcer disease (PUD) prophylaxis; and deep venous thrombosis (DVT) prophylaxis. Results: Among a total of 2747 patients, the bundle compliance rate in January 2010 was 30%, and reached to 100% in December 2010, while the overall rate was 78.9%. The individual bundle compliance rates were as follows: head-of-bed elevation - 99.9%; daily sedation vacation - 88.9%; PUD prophylaxis - 94.9%; and DVT prophylaxis - 85.7%. At the beginning, VAP rate was 2.5/1000 ventilator days, and reduced to 0.54 in the next month. The overall VAP incidence rate in 2010 was found to be 1.98 with a reduction of 1.41 by comparing with the same data of year 2009 collected retrospectively. The total reduction cost in one year was $154,930. A significant correlation was found between the VAP rate and its bundle compliance (p=0.001). Most frequent pathogens found were Pseudomonas aeruginosa (30.8% of all isolates) followed by Acinetobacter baumannii (27.7%), and methicillin-resistant Staphylococcus aureus (15.4%). Conclusion: Application of VAP prevention bundle reduced the VAP incidence rate and lowered the cost of care.
3. Ashshi AM (2012). ‘Detection of occult hepatitis B virus in anti-HBc positive/anti-HBs positive blood donors in Saudi Arabia.’ Res J Med Sci; 6: 61-65.
http://www.medwelljournals.com/abstract/?doi=rjmsci.2012.61.65
(Laboratory Medicine Department)
ABSTRACT
Despite the progress made in the prevention of transfusion transmitted infections, transmission of Hepatitis B Virus (HBV) from donors with Occult Hepatitis B virus (OHB) still poses a challenge to the safety of blood donation. The presence of OHB in anti-hepatitis B core antigen (anti-HBc) positive/anti-hepatitis B surface antigen (anti-HBs) negative blood donors has recently been documented worldwide however, its prevalence in anti-HBc positive/anti-HBs positive donors remains obscure. Therefore, this study was aimed to evaluate the presence of OHB among anti-HBc/anti-HBs-positive blood donors. To meet these objectives, a total of 1000 serum samples of consenting blood donors negative for Hepatitis B surface Antigen (HBsAg) were collected at the Hera’a General Hospital, Makkah Al-Mukaramah, Saudi Arabia and screened for anti-HBs and anti-HBc antibodies. According to their serological status, the samples were classified into anti-HBc negative/anti-HBs negative; anti-HBc positive/anti-HBs negative and anti-HBc positive/anti-HBs positive donors. Samples that showed positive reactivity for both anti-HBc/anti-HBs antibodies were then selected for quantitative detection of HBV DNA by using RT-PCR. Results showed that of the 1000 HBsAg-negative donors, 12 (1.2%) were anti-HBc positive/anti-HBs negative and 63 (6.3%) were anti-HBc positive/anti-HBs positive. Additionally, among these 63 anti-HBc positive/anti-HBs positive blood donor population HBV DNA was detected in two of them (3.2%). In conclusion, the results showed the presence of HBV DNA in the sera of anti-HBc positive/anti-HBs positive blood donors in Saudi Arabia. Overall, these results can raise an important public health issue that an anti-HBc positive/anti-HBs positive serum is not a sign of total HBV eradication and in turn, highlight the need for a stringent and better screening system to prevent post-transfusion HBV infection.
4. Ashshi AM (2012). ‘Is Rotavirus Infection Still Responsible for Acute Gastroenteritis and Severe Diarrhea among Children in Holy Makkah?’ Research Journal of Medical Sciences; 6: 170-174.
http://www.medwelljournals.com/abstract/?doi=rjmsci.2012.170.174
(Laboratory Medicine Department)
ABSTRACT
Acute gastroenteritis and severe diarrhea is a leading cause of preventable death in infants and young children worldwide. It ranks second to neonatal deaths as the major cause of childhood mortality. In this regard, rotavirus infection is the most important microbial causative agent, particularly in developing countries. Coherently, this study was designed to assess the prevalence of rotavirus infection among young children with acute gastroenteritis and severe diarrhea in Holy Makkah, Saudi Arabia and evaluate the specificity of Latex Agglutination Test (LAT) in detection of rotavirus infection in comparison with Reverse-Transcription Polymerase Chain Reaction (RT-PCR). During the period from March to September 2011, stool samples were collected from 100 children (of both sex and under 5 years of age) with acute gastroenteritis and severe diarrhea and admitted to the pediatric wards at the Garwal Hospital in Holly Makkah. For detection of rotavirus infection, the samples were first examined by LAT and then by RT-PCR using specific primer sequences for human rotavirus VP4 and VP7 genes. Sixteen samples of these 100 samples (16%) were positive for rotavirus by LAT. However, the genetic materials of rotavirus (VP4 and VP7 genes) were only detected in 4 samples of these 100 samples (4%) by RT-PCR amplification. The samples that tested negative with LAT were also negative with RT-PCR. Overall, this study indicated that rotavirus infection still responsible for acute gastroenteritis and severe diarrhea among children in Holy Makkah with a prevalence of 4%. In turn, this may help in assessing the success of the rotavirus vaccine in the future. In addition, this study reflects the low specificity of LAT than RT-PCR for detection of rotavirus infection.
5. Azzeh FS (2012). ‘Camel Milk as Functional Food: Review Paper.’ (In Arabic). Arab Journal of Food and Nutrition; 29: 94-111.
http://ageconsearch.umn.edu/bitstream/159395/2/Camel%20Milk%20Technology%20Development.pdf
(Clinical Nutrition Department)
ABSTRACT
None
6. Azzeh FS (2012). ‘Relationship between Vitamin D and Rheumatoid Arthritis Disease: Review Paper.’ Pakistan Journal of Nutrition, 11: 293-300.
(Clinical Nutrition Department)
ABSTRACT
Rheumatoid arthritis is a chronic inflammatory disease in which the synovial membrane of the joint becomes inflamed, resulting in a swelling, stiffness, pain, limited range of motion, joint deformity and disability. Vitamin D is the “sunshine vitamin” which is converted in the body to a hormone 1, 25- dihydroxyvitamin D3 by the photolytic action of ultraviolet light on the skin. Vitamin D plays an important role, along with the essential minerals calcium and phosphorus, in the maintenance of healthy bones and teeth. An inverse association between vitamin D intake and rheumatoid arthritis was found in some prospective studies. Although vitamin D has been implicated in a decreased risk of autoimmune diseases such as type 1 diabetes and multiple sclerosis, its role in decreasing the risk of rheumatoid arthritis remains equivocal. This scientific review is written to illustrate the possible relationships between rheumatoid arthritis and vitamin D.
7. Bukhari HM, Abdelghany HA, Nada IS and Header EA (2012). ‘Effect of yoghurt pillared with propolis on hyperglycemic rats.’ Egyp J Hospit Med, 49:691-704.
http://www.oalib.com/paper/2801375
(Laboratory Medicine & Clinical Nutrition Departments)
ABSTRACT
Dietary supplementation of yoghurt with plants rich in antioxidants such as propolis which is an adhesive resinous material collected by honey bees is recently recommended. This study aimed at investigating the protective effect against the hyperglycemia and hyperlipidemia. The study showed that yoghurt with propolis had a hypoglycemic effect together with reduction of the serum levels of cholesterol, low density and very low density lipoproteins with elevation of high density lipoproteins. The atherosclerosis indices were affected with reduction of low density lipoproteins-cholesterol/high density lipoproteins-cholesterol and elevation of the high density lipoprotein-cholesterol/total cholesterol. This was attributed to the reduction of glucose absorption and inhibition of α-glucosidase together with the antioxidant activity with regain of the pancreatic β-cell function. The hypolipidemic effect and consequently protective effect against atherosclerosis was attributed to the binding, breakdown and reduction of absorption of cholesterol together with the antioxidant activity of both yoghurt and propolis. Down regulation of the expression of the angiogenic gene factors which have a role in the pathogenesis of atherosclerosis may play a role. Thus, this dietary supplementation may be manufactured and used for its value in reducing hyperglycemia and hyperlipidemia in cases of diabetes mellitus.
8. Bukhari HM, Nada IS and Header EA (2012). ‘Effect of obesity and dietary factors on bone mineral density levels among female students in Umm Al-Qura University.’ Egyp J Hospit. Med; 49:678-690.
http://egyptianjournal.net78.net/49_13.pdf
(Clinical Nutrition & Laboratory Medicine Departments)
ABSTRACT
Background: Nutrition is one of the most important factors influencing human health. Also, nutrition plays a role in the etiology of osteoporosis disease. This disease is a serious metabolic bone disorder that often results in hip fracture and is usually asymptomatic in its initial stages. Objective: Assess the prevalence of osteoporosis among female university students. Methods: A cross sectional study was carried out during the period from 1/1/2010 to 30/6/2010 among a random sample of (257) university female students were chosen from Umm Al Qura of Makkah. The age of sample from 19-24 years old. Data were collected through an interview with case by using a special questionnaire; bone mineral density (BMD) and body composition have also been measured. Results: Osteoporosis was present in 7% of cases while, osteopenia was current in 32.3% of cases. Moreover there was a highly positive significant relationship at level (1%) between osteoporosis induced and each of body fat %, fat weight, and BMI. Conclusion: The prevalence of osteoporosis among university students was positively and significantly associated (p<0.001) with increased body fat. The study results suggested that inevitable decrease in body fatness and weight with less consumption of carbonated beverages, taking into consideration variety and balanced diets and increasing nutrition education programs will improve bone health and nutritional status.
9. Bukhar HM, Bakri EH, Ali H, and Header EA (2012). ‘Hepatorenal Protective Effect Of some legumes On Normal And Hyperglycemic Rats.’ Home Econ. J.; 22.
(Clinical Nutrition Department)
ABSTRACT
Diabetes mellitus is a chronic disease that has affected humankind throughout the world. The records of the ancient civilizations of Egypt, India, Japan, Greece, and Rome described the symptoms of the disease. The use of traditional plant for diabetes mellitus is widely practiced in Middle East countries. This investigation aimed to study hepato-protective effect of (lupines, fenugreek, and snap beans) on hyperglycemic rats. Forty eight male white albino rats weighing 165.5±2.5g were used in this study and fed basal diet for 1 week for adaptation, 42 of rats injured by diabetes with a single intraperitoneal injection of alloxan at dose level of 150 mg/kg body weight. Rats divided into 8 groups (6 rats/each group). Six normal and 6 diabitic rats fed on basal diet, while other 6 diebtic groups fed on 15 or 25 % lupines, fenugreak or snap beans diets. At the end of experiment period 45 days, the weight gain was calculated, blood samples were collected and liver of each rat was removed rapidly and weighted separately. Blood samples were used for estimation of fasting serum glucose, fasting serum ALT, AST and ALP and serum lipid profile. Data arrived at showed that serum AST and ALT levels declined significantly (p < 0.05) in all treated groups that fed on 15% and 25% (lupines, fenugreek, and bean) when compared with diabetic control positive group. Oral administration of plant decreased histopathological changes in the giver. Lupines, fenugreek, and bean appear to improve not only the hyperglycemia but also associated dyslipidemia and seem to have a hepatoprotective effect in alloxan-induced diabetic rats.
10. Ebid AA, El-Shamy SM, Thabet AA (2012). ‘Effect of Posterior Tibial Nerve Stimulation and Trospium Hydrochloride in Treatment of Overactive Bladder Syndrome: a randomized controlled study.’ Indian Journal of Physiotherapy and Occupational Therapy; 6: 36-41.
http://www.i-scholar.in/index.php/ijpot/article/view/48396
(Physiotherapy Department)
ABSTRACT
Objective: To compare the effects of long term posterior tibial nerve electrical stimulation and trospium hydrochloride on urodynamic parameters, bladder diary and severity of urgency in patients with overactive bladder syndrome. Design: Randomized controlled trial. Subjects: Thirty-seven patients were divided into either posterior tibial nerve electrical stimulation (Group 1) or trospium hydrochloride (Group 2). Main outcome measures: All patients were assessed at the beginning of the treatment, at week 12 (end of treatment), 18 and 24 according to urodynamic parameters, voiding diary parameters and severity of urgency (visual analogue scale VAS). Results: Statistically significant improvements were observed in both groups according to some urodynamic parameters, voiding diary parameters, VAS urgency severity at the end of the treatment. During the 24-week follow-up period, deteriorations were observed in many parameters in both groups although improvements in the volume at first desire to void, frequency of urgency and VAS urgency severity in group 1 persisted. Significant differences were not detected between groups at the end of the treatment or during the post treatment follow-up controls. Conclusion: No difference was detected in long term of posterior tibial nerve electrical stimulation and trospium hydrochloride in the treatment of patients with overactive bladder syndrome. Discontinuation of both treatments caused deterioration in most of the symptoms of overactive bladder syndrome.
11. Elmadbouly MA and Abd Elhafez AM (2012). ‘Assessment of Nutritional Status of Hospitalized Elderly Patients in Makkah Governorate.’ Pakistan Journal of Nutrition; 11: 984-990.
http://www.pjbs.org/pjnonline/fin2410.pdf
(Clinical Nutrition Department)
ABSTRACT
A cross sectional study was conducted to assess the nutritional status of a sample of 102 recently hospitalized elderly patients in general hospitals at Makkah Governorate by using the Mini Nutritional Assessment (MNA) tool. Among the studied patients, 22.6% were classified as malnourished, 57.8% were at risk of malnutrition and 19.6% were as well nourished. Low body mass index, history of weight loss during the last 3 months, living independently, taking > 3 prescription of drugs per day and presence of neuropsychological problems were associated with malnutrition (p<0.05). The elderly classified as malnourished and those at risk of malnutrition and well-nourished differed significantly in all dietary assessment variables of the MNA score (p<0.05). It is concluded that malnutrition is a common problem among older people. MNA test should be performed for elderly upon admission to hospitals and more attention need to be paid to this vulnerable group of people.
12. Kensarah OA and Azzeh FS. (2012). ‘Vitamin D Status of Healthy School Children from Western Saudi Arabia.’ Pakistan Journal of Nutrition, 11: 288-292.
(Clinical Nutrition Department)
ABSTRACT
This study was conducted to evaluate vitamin D in healthy school children from Makkah area. A total of 148 healthy school children (87 boys and 61 girls) were included in this study. Vitamin D as 25- hydroxyvitamin D [25 (OH)-D] was measured as indication of vitamin D status. Serum 25 (OH)-D less than 20 ng/ml was considered as vitamin D deficient subject. Other biochemical indicators were also measured as calcium, phosphorus, alkaline phosphatase and parathyroid hormone. The mean age of the subjects was 10.1 years. Overall serum vitamin D was 10.5 ng/ml, which was significantly higher in male group than female. About 96.7% and 78.2% of females and males were deficient in vitamin D, respectively. No sufficient vitamin D female subject was recognized. The prevalence of vitamin D deficiency in children school from Makkah was very high and the deficiency was higher in females than males. The main reason for vitamin D deficiency was the restriction to sunlight exposure.
13. Kensarah OA and Azzeh FS (2012). ‘Implementing High Vitamin C Treatments to Decrease Blood Uric Acid Levels in Hyperuricemic Saudi Patients.’ Journal of American Science; 8: 462-467.
(Clinical Nutrition Department)
ABSTRACT
This study was conducted to determine the effects of high vitamin C intake from diet and supplements on serum uric acid concentrations during 2 months. A group of 30 Saudi adults, non-smokers, hyperurecemic, from both genders and aged between 20-70 yrs. Participants were divided into 3 groups; control group (low purine diet with normal vitamin C intake), high dietary vitamin C with purine restricted diet and high vitamin C supplements with purine restricted diet. The high vitamin C dose was 500mg/day. All participants have been followed-up for two months and reviewed at least two times a week. Uric acid, creatinine and estimated glomerular filtration rate were measured before and during the study. The overall mean reduction of uric acid for dietary treated group was -0.77 mg/dl and for supplemented group was -0.28 mg/dl. In the control group, the average uric acid was incremented after 2 months by0.51 mg/dl. Reduction in serum uric acid was statistically significant for dietary treated group but not for supplemented one. This study suggests that inclusion of 500 mg/day of vitamin C for 2 months reduced risk factors associated with hyperuricemia. Dietary treatment was more effective in reduction serum uric acid than supplements.
14. Kensara OA, ElSawy NA, and Header EA (2012). ‘Aqueous Extract of Thymus Vulgaris-induced Prevention of Kidney Damage in Hypertensive Adult Male Albino Rat: Biochemical and Ultrastructural Study.’ Pakistan Journal of Nutrition; 11: 367-374
http://www.pjbs.org/pjnonline/fin2269.pdf
(Clinical Nutrition & Laboratory Medicine Departments)
ABSTRACT
Different metabolic disorders including hypertension cause renal damage. The aim of this study was to study the effect of Thymus Vulgaris (TV) on kidneys of induced hypertensive rats through biochemical and ultrastructure examination. Thirty three adult male albino rats were divided into 3 groups 11 animals each (control, untreated and treated). The first (control) group was fed and followed up for 8 weeks by measuring the systolic BP. In the second group, hypertension was induced by ligation of left renal artery to have systolic BP >150 mmHg and was followed up till the end of study. The third group in which hypertension was induced were given TV as 100 mg/kg body weight daily. The surviving 11 rats per each group were weighed again and killed with an overdose of phenobarbital and blood was collected to measure the blood chemistry. Following laparotomy, right kidney was dissected and excised from each rat and divided into 2 parts: one part was immersed in 10% formal saline and prepared for light microscopic examination. The other parts was immersed into 2.5 % glutaraldehyde and prepared for electron microscopic examination using transmission microscopy in faculty of medicine Zagazig university Egypt. A Significant increase was noticed in creatinine and cholesterol in untreated in contrast to control and treated hypertensive rats. There was significant reduction of Blood Pressure (BP) on using TV in contrast to untreated group. In untreated hypertensive group some cortical fields showed scars surrounding obsolete shrinkage glomeruli with inflammatory infiltrates with reciprocal dilatation of their Bowman's capsules. Ultrastructure examination showed thick glomerular endothelium with obliterated endothelial fenestrations. In treated hypertensive group the rat kidneys showed preserved cortical appearance and normal trilaminar structure but with focal thickening. Induced hypertension in rats affected renal tissues with biochemical alterations which all can be improved and/or prevented by using Thymus vulgaris herbal extract.
15. Bukhar HM, ElSawy NA, and Header EA (2012). ‘Biological effect of high energy drink on normal and hyperglycemic rats.’ Pakistan Journal of Nutrition; 11: 301-309.
http://www.jofamericanscience.org/journals/am-sci/am0808/105_8891bam0808_688_697.pdf
(Clinical Nutrition & Laboratory Medicine Departments)
ABSTRACT
Several studies suggest that there was relationships between energy drink consumption and problem behaviors among adolescents and adults as it increase lipolysis glycogenolysis and catecholamine secretion. This study aimed to find out the potential effects of high energy drinks recommended intake and toxic dose on normal and hyperglycemic rats. Thirty-six (36) male adult Sprague-Dawley rats weighting 145±5.3 g each were used in this investigation. Non-diabetic rats [control-ve 6 rats feed on basal diet only and 12 Normal Rats (NR) divided into two groups consumed basal diet with 1 and 2 ml of High Energy Drink (HED) by gastric tube], while Diabetic Rats (DR) control+ve 6 rats received basal diet only and 12 rats divided into two groups consumed basal diet with 1 and 2 ml of HED after injected with alloxan for inducing diabetes mellitus. Body Weight Gain (BWG) and food intake were recorded weekly for 6 weeks. Blood samples were collected after 12 hours fasting at the end of experiment. Liver was removed and weighted. Blood serum was prepared for measurements of glucose, triglyceride, cholesterol, HDL-c, LDL-c, VLDL-c, AST, ALT and ALP. The BWG of NR groups received 2 ml only and DR groups received 1 and 2 ml of HED by oral injection recorded significant decrease (p<0.001) as compared to the control negative group. Blood glucose level was significantly higher (p<0.001) for DR fed on 1 and 2 ml compared with control (-). Serum AST, ALT and ALP were significantly higher (p<0.01 and p<0.001 resp.) for NR received the two doses of HED compared with normal rats control (-). As for cholesterol, triglycerides and LDLc levels were significantly higher (p<0.01) in the hyperglycemic rats group fed on 2 ml of HED compared with control (-). Also LDLc/HDLc ratio increased gradually when the level of HED increased. Oral injection by HED cause histopathological changes in the liver for NR and DR like atrophy and cell damage also changes in the chemical and morphological structure.
16. El-Shemi AG and Faidah H (2011). ‘Synergy of daptomycin with fusidin against invasive systemic infection and septic arthritis induced by type VI group B streptococci in mice.’ Afr. J. Pharm. Pharmacol.; 5:1125-1131.
http://www.academicjournals.org/journal/AJPP/article-full-text-pdf/A4D07FC33137
(Laboratory Medicine Department)
ABSTRACT
In view of the emergence of multidrug-resistant group B streptococci (GBS), and its significant clinical impact, there is a necessary need for the development of more effective therapeutic alternatives. Here, we assessed the therapeutic efficacy of daptomycin, a novel lipopeptide antibiotic, in the treatment of type IV GBS-induced invasive systemic infection and septic arthritis in mice. We also evaluated the possible synergy between daptomycin and fusidin to combat GBS disease. Mice infected with type IV GBS and left without drug treatment displayed high incidence of deaths and severe diffuse septic arthritis, associated with excessive production of proinflammatory cytokines (tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6)), cyclooxygeanse-2 (COX-2) and prostaglandin E2 (PGE2) in their blood and joints. However, treatment of these GBS-infected mice with daptomycin significantly inhibited the inoculated bacteria to grow in the blood and joints. Daptomycin treated mice had significantly showed lower mortality rates, less frequent arthritis and lower levels of TNF-α, IL-1β, IL-6, COX-2 and PGE2 than infected untreated animals. More interestingly, a marked in vivo synergy between daptomycin and fusidin that completely protected the mice from GBS infection and its associated mortality and serious sequels was clearly observed. In summary, the present study showed that daptomycin is a welcome newcomer antibacterial arsenal to eradicate GBS invasive infection and septic arthritis, in particular when given in combination with other antibacterial agents such as fusidin.
17. El-Shemi AG, Basalamah MA, Kensara OA, Ashshi AM (2011). ‘Interleukin-22 therapy attenuates the development of acute pancreatitis in rats.’ J. Clin. Med. Res. 3:82-88.
(Laboratory Medicine & Clinical Nutrition Departments)
ABSTRACT
Human acute pancreatitis (AP) is a potential serious disease with a limited specific therapy. Here, we assessed the therapeutic efficacy of interleukin-22 (IL-22), a newly emerged cytokine with unique biological activities, in the treatment of a rat model of severe AP. For induction of AP, rats were intraperitoneally (i.p.) injected with nine doses of caerulein (50 μg/kg/dose) at 1-h intervals. Recombinant rat IL-22 (rIL-22) was given in three doses (4 μg/rat/dose; i.p.) after the 1st, 5th, and 9th caerulein injection. Serum levels of pancreatic α-amylase and lipase, pancreatic weight/body weight ratio, histological score of pancreatic injury, intrapancreatic expression of myeloperoxidase (MPO; as an index of neutrophils infiltration) and cyclooxygenase-2 (COX-2), and the serum and pancreatic levels of pro-inflammatory prostaglandin E2 (PGE2); monocyte chemotactic protein-1 (MCP-1); and Interleukin-1β (IL-1β) were collectively analyzed as diagnostic parameters of the induced AP. Results showed that administration of rIL-22 significantly repressed caerulein-evoked substantial hyperamylasemia and hyperlipasemia, severe pancreatic injury and edema. Also the administered rIL- 22 significantly reduced caerulein-induced intrapancreatic overexpression of COX-2 and MPO, and the production of proinflammatory mediators (PGE2, MCP-1 and IL-1β) in the systemic circulation and pancreatic tissues. Taken together, these results indicate the potential therapeutic efficacy of IL-22 against AP by acting as an anti-inflammatory agent.
18. Sheweita SA, Yousef MI, Baghdadi HH, Elshemy AG (2012). Changes of drug metabolizing enzymes in the liver of male sheep exposed to either cypermethrin or dimethoate. Drug Metab Lett. 6:2-6.
http://www.eurekaselect.com/97780/article
(Laboratory Medicine Department)
ABSTRACT
Xenobiotics such as insecticides are metabolized to more or less toxic metabolites by drug-metabolizing enzymes including cytochrome P450 (Cyp P450), cytochrome b5 (Cyp b5), NADPH-cytochrome c reductase (Cyt.c R), N-nitrosdimethylamine-N-demethylase I (NDMA-dI), glutathione (GSH), glutathione s-transferase (GST), and glutathione reductase (GR). Therefore, the present study showed the influence of oral administration of cypermethrin (6 and 12 mg/kg/day) and dimethoate (1.6 and 3.2 mg/kg/day) for 63 consecutive days on the activities of the above mentioned enzymes in the livers of male sheep. Low and high-treatments of sheep with cypermethrin significantly increased the levels of Cyp P450 by 56% and 98%, Cyp b5 by 65% and 80%, GSH by 68% and 74%, and Cyt.c R by 67% and 98%, respectively in a dose-dependent manner. However, low dose of cypermethrin increased the activities of GST and GR by 56% and 91% respectively. In addition, low and high dose-treatments with dimethoate increased the hepatic contents of Cyp P450 by 27% and 40%, GSH by 259% and 132%, whereas NDMA-dI decreased by 27 and 55% respectively, and no change in the content of Cyp b5 and the activity of Cyt.c-R at any given dose of this compound. It is concluded that exposure to cypermethrin and dimethoate significantly changed the hepatic activity of phases I & II drugmetabolizing enzymes in sheep, and these changes are mainly dependent on the administred dose, and also on the type of the tested insecticides. Also, such changes should be considered when therapeutic drugs administered to people exposed to such insecticides.
19. Thabet AA, Helal OF, El-Shamy SM (2012). ‘Efficacy of Low-Intensity Pulsed Ultrasound on Bone Mineral Density in Osteoporotic Postmenopausal Women.’ Indian Journal of Physiotherapy & Occupational Therapy; 6: 238-242.
(Physiotherapy Department)
ABSTRACT
Purpose of this current study was to investigate the effect of low intensity pulsed ultrasound on bone mineral density (BMD) of lumbar vertebrae in osteoporotic postmenopausal women. Thirty postmenopausal women suffer from lumbar vertebrae osteoporosis were selected from Umm El Masryeen General Hospital received ultrasound therapy three time / week for six weeks. DXA was used for assessment of BMD before and after treatment. The results of the study demonstrate significant increase in lumbar BMD. It was concluded that low intensity pulsed ultrasound is effective modalities in increasing bone mineral density (BMD) of lumbar vertebrae in osteoporotic postmenopausal women.
20. El-Shamy SM, Abdelaal AAM and Helal OF (2012). ‘Ventilatory response to aerobic exercise in obese children.’ Bioscience Research 9: 35-40.
http://www.isisn.org/BR%209(1)%202012/35-40%209(1)%202012%20BR-904.pdf
(Physiotherapy Department)
ABSTRACT
This study was conducted at the Faculty of Physical Therapy, Cairo University, Egypt in 2010, to examine the effects of aerobic exercise in ventilatory functions in obese children. Forty obese children and 20 age and sex matched non obese control group were studied. Their age was ranging from 10 to 14 years. They were randomly assigned to study and control group. A child with a body mass index (BMI) in the 95th percentile or greater was considered to be obese. The study group (A) received aerobic exercise training on treadmill while children in the control group (B) did not participate in any intervention for 3 months. Pulmonary function test (FVC, FEV1, FEV1/FVC, MMEFR, and MBC) was used to measure lung functions in all children pre and post treatment. The results of this study revealed no significant difference was recorded between the two groups before treatment. While after 3 months, significant improvement was recorded in the two groups. There was also significant difference between the two groups in favor of the study group. From the obtained results, it can be concluded that, aerobic exercise training program had positive effects on lung functions in obese children.
21. Elsayed GM, Ismail MM, and Moneer MM (2011). ‘Expression of P-glycoprotein, Cyclin D1 and Ki-67 in Acute Lymphoblastic Leukemia: Relation with Induction Chemotherapy and Overall Survival.’ Indian Journal of Hematology and Blood Transfusion; 27: 157-163.
http://link.springer.com/article/10.1007%2Fs12288-011-0086-y
(Laboratory Medicine Department)
ABSTRACT
Previous studies showed that non-cycling cells have a higher multidrug resistance (MDR) expression, which may be down-regulated by proliferation induction. Triggering these cells into proliferation down-regulates high MDR expression. The aim of this study was to determine the expression of P-glycoprotein (PGP) and cell cycle parameters (cyclin D1 and Ki-67) in acute lymphoblastic leukemia (ALL) at diagnosis, and to evaluate the correlation between the expressions of each marker, and the clinical significance of such expression with response to induction chemotherapy and overall survival. A total of 78 newly diagnosed ALL patients were enrolled in our study. PGP, cyclin D1 and Ki-67 were determined by flow cytometry. PGP expression was encountered in 10/78 (12.8%) of ALL cases. Cyclin D1 and Ki-67 were expressed in 16/77 (20.6%) and 27/76 (34.6%) of ALL cases, respectively. None of the parameters were associated with response to induction chemotherapy and overall survival. Based on the current analysis, we conclude that a joint immunophenotypic evaluation of PGP and cell cycle parameters like that adopted in this study is unlikely to reveal mechanisms of multidrug resistance associated with the clinical outcome.
22. Mohamed A, Abou Elella G, Nasr E, and Soliman Y (2012). ‘Evaluation of Fibronectin-Binding Protein Ag85-B as Target for Serodiagnosis of Swine Mycobacteriosis in Living Animals.’ J Mycobac Dis S; 2: 2161-1068.
(Laboratory Medicine Department)
ABSTRACT
The aim of the current study was to evaluate the fibronectin-binding protein Ag85-B as a potential antigen for ELISA-based serodiagnosis of swine mycobacteriosis in living pigs. The validity of Ag85-B, in comparison with tuberculin purified protein derivatives (PPD), for the serodiagnosis of swine mycobacteriosis was investigated in the current study. A total of 89 serum samples from previously confirmed Mycobacterium and non-Mycobacterium infected slaughtered pigs were used for the evaluation study. These included 66 serum samples from confirmed cases with swine mycobacteriosis and 23 samples from non-Mycobacterium infected cases. Obtained ELISA results revealed an overall accuracy index, sensitivity, specificity, positive predictive value, and negative predictive value of 87.6%, 87.9%, 86.9%, 95.1%, and 71.4%, respectively for Ag85-B as compared to 80.9%, 86.4%, 65.2%, 87.7%, and 62.5%, respectively for tuberculin PPD. In conclusion, the study revealed the reliability of Ag85-B as a potential candidate for ELISA-based serological assay that would provide for early and rapid diagnosis of swine mycobacterial infections in living animals.
23. Mohamed A, Abdel-Rady A, Ahmed L, and El-Hosary A (2012). ‘Evaluation of indirect TaSP enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in Egypt.’ Veterinary parasitology; 186: 486-489.
http://www.sciencedirect.com/science/article/pii/S0304401711007606
(Laboratory Medicine Department)
ABSTRACT
The aim of the present study was to evaluate the validity of Theileria annulata surface protein (TaSP)-ELISA, in comparison with traditional microscopic test, for the diagnosis of T. annulata infection among Egyptian baladi cattle (Bos taurus) and water buffaloes (Bubalus bubalis). Molecular confirmation of infection using T. annulata merozoite surface (Tams-1) target amplification by PCR was used as a gold standard. A total of 76 clinically suspected animals including 64 baladi cattle and 12 water buffaloes were investigated in the current study by the three methods. Based on the PCR-confirmed results, the evaluation study revealed higher sensitivity of TaSP-ELISA (72.9% and 75%) as compared to microscopic examination (58.3% and 50%) among cattle and buffaloes, respectively. On the other hand, the specificity of TaSP-ELISA in diagnosis of T. annulata infection was higher (87.5%) in baladi cattle as compared to water buffaloes (37.5%). In conclusion, TaSP-ELISA was shown to be suitable for the diagnosis of T. annulata infection in cattle under field conditions.
24. Ali MME, El-Megeid AAA and Mostafa RAA (2012). ‘Effect of some levels from ginseng, barley and carob on lipid profile and kidney functions of rats fed on high fructose diet.’ Journal of American science; 8: 152-162.
http://www.jofamericanscience.org/journals/am-sci/am0810/023_10724am0810_152_162.pdf
(Clinical Nutrition Department)
ABSTRACT
The main target of the present investigation is to study the effect of three levels from ginseng, barley and carob (2.5%, 5% and 7.5%) and their combinations on biological value, lipid profile and kidney functions and histopathological examination in kidney of rats fed on high fructose diet. Seventy male Sprague albino rats divided into two main groups. The first group fed on basal diet as a (-ve control group), while the second main group fed on high fructose diet for two weeks. The second main group divided into thirteen subgroups. Subgroups (1, 2&3) fed on high fructose diet (HFD) containing 2.5%, 5% and 7.5% ginseng, respectively. Subgroups (4, 5&6) fed on HFD containing 2.5%, 5% and 7.5% barley, respectively. Subgroups (7, 8 & 9) fed on HFD containing 2.5%, 5% and 7.5% carob. Subgroups (10, 11&12) fed on HFD containing 2.5%, 5% and 7.5% combination of (ginseng, barley and carob). Subgroups (13) fed on HFD only (control positive group). At the end of the experimental period (4 weeks) rats were fasted over night before sacrificing, blood was collected then centrifuged to separate the serum. Kidney was removed from each rat, cleaned and weighted to estimate of kidney weight / body weight percent. Kidney was examined hestopathologically. The obtained results revealed that, feeding rats on high fructose diet led to significant increase in (body weight gain %, kidney weight/body weight %, cholesterol, triglycerides, LDL-c, VLDL-c, uric acid, urea nitrogen, creatinine) and decreased (food intake and HDL-c). Feeding rats on high fructose diet with the different levels from ginseng, barley, carob and their combinations improved all parameters and kidney weight, especially when used the high level from (barley, ginseng and combination of ginseng, barley and carob). The histopathological examination in the kidney confirmed this improvement.
25. Refaat B and Ledger W (2011). ‘The expression of activins, their type II receptors and follistatin in human Fallopian tube during the menstrual cycle and in pseudo-pregnancy.’ Human reproduction; 26: 3346-3354.
http://humrep.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=21984573
(Laboratory Medicine Department)
ABSTRACT
Background: The Fallopian tube (FT) is the site of fertilization and early embryonic development. We have previously reported the expression of activins, their receptors and follistatin by the FT. Here, our aim was to study the expression of tubal activins, their type II receptors and follistatin during the menstrual cycle and following exposure to hCG in vivo. Method: A set of 30 FTs were collected from cycling women (n = 12) at different stages of the cycle (n = 4 in each stage) and pseudo-pregnant women (n = 3) at the time of hysterectomy for benign disease. The pseudo-pregnant women were injected with hCG in the days leading up to hysterectomy, and pseudo-pregnancy was confirmed by the persistence of amenorrhea, the presence of corpus luteum and decidualization of the endometrium. FT specimens were examined using immunohistochemistry and quantitative RT-PCR. Results: The expression of activin βA- and βB-subunits, activin type IIA and IIB receptors, and follistatin varied throughout the menstrual cycle, being lowest in the follicular phase and highest in the luteal phase. These results were demonstrated at the mRNA and protein level by quantitative RT-PCR and immunohistochemistry (P< 0.05). HCG injection rescued the expression of the candidate molecules from falling to the follicular stage levels but the expression remained lower than in the luteal phase. Conclusions: We suggest that activins play a role in tubal physiology and early embryonic development. Additionally, exposure of the tubal epithelium to hCG modulates the expression of tubal activins.
26. Refaat B, Simpson H, Britton E, Biswas J, Wells M, Aplin JD, and Ledger W (2012). ‘Why does the fallopian tube fail in ectopic pregnancy? The role of activins, inducible nitric oxide synthase, and MUC1 in ectopic implantation.’ Fertility and sterility; 97: 1115-1123.
http://www.fertstert.org/article/S0015-0282(12)00269-5/abstract
(Laboratory Medicine Department)
ABSTRACT
OBJECTIVE: To investigate the role of activin-βA subunit, activin type II receptors, inducible nitric oxide synthase (iNOS), and MUC1 in the pathogenesis of ectopic pregnancy (EP) and their involvement in the determination of the implantation site. DESIGN: Observational study. SETTING: Academic unit of reproductive and developmental medicine. PATIENT(S): Four women at the luteal phase, three pseudopregnant women at the time of hysterectomy for benign disease, and 10 archived cases of EP. We collected 14 Fallopian tubes were collected from four women at the luteal phase and three pseudopregnant women at the time of hysterectomy for benign disease; specimens from implantation site, trophoblast and remote sites from the implantation site were collected from 10 archived cases of EP. INTERVENTION(S): Immunohistochemistry and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR). MAIN OUTCOME MEASURE(S): Comparison of the expression of candidate molecules between the different groups. RESULT(S): The expression of activin-βA subunit, activin type II receptors, and iNOS was statistically significantly increased and expression of MUC1 statistically significantly decreased in tubes bearing an EP. There was no statistically significant difference in the expression of the candidate molecules between the implantation and remote sites. Candidate molecules were also expressed in the trophoblast. CONCLUSION(S): The pathological expression of candidate molecules by tubes bearing an EP is not involved in the determination of implantation site. Additionally, candidate molecules may play a role in the regulation of trophoblast cells in vivo during early pregnancy.
