جامعة أم القرى

جامعة أم القرى

المجلد - 7 ، الإصدار -1: يونيو ، 2021 / مقالة - سلعة 2


- 2022/04/14

Effect of Carcinoembryonic Antigen-related Cell Adhesion Molecule-binding Recombinant Polypeptide on the Killing of Neisseria meningitidis by Human Neutrophils

 

Abdel-Rahman Youssefa,b,c*
Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al-Qura University, Makkah, Saudi Arabia.
Department of Microbiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
School of Cellular & Molecular Medicine, University of Bristol, Bristol, UK.
Volume: 7 | Issue: 1 | Pages: 6-9 | June 2021 | https://doi.org/10.54940/ms10183116 PDF

Abstract

Background: Neutrophils are an essential part of innate immunity and play a crucial role in controlling infection caused by Neisseria meningitidis. The Moraxella catarrhalis ubiquitous surface protein A1 (UspA1)-based recombinant polypeptide binds to the carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) 1 receptor on host cells and blocks binding of the mucosal pathogens to human epithelial cells and T cells. Aim of the study: Since the CEACAM1 receptor is expressed on neutrophils, the aim of this study was to investigate the effect of CEACAM1-binding recombinant polypeptide on the ability of neutrophils to kill Neisseria meningitidis.
Methods: The effect of CEACAM1-binding recombinant polypeptide on the phagocytic killing of Neisseria meningitidis by neutrophils was assessed by incubation of neutrophils with CEACAM1-binding recombinant polypeptide (UspA1 527–665) or with CEACAM1-non-binding polypeptide control (UspA1 659–863) for one hour before infection with Neisseria meningitidis. The surviving bacteria were released and counted. Results: 30 minutes after infection of neutrophils with Neisseria meningitidis, the survival of bacteria in presence of CEACAM1-binding recombinant polypeptide was 64% compared to 52% with control peptide and 43% without peptide. However, one-hour after infection, the surviving bacteria was 32% in presence of CEACAM1-binding recombinant polypeptide compared to 18% with control peptide and 22% without peptides.
Conclusion: Although CEACAM1-binding polypeptide reduced the killing of Neisseria meningitidis by neutrophils, it did not entirely stop phagocytosis and killing of bacteria.

Keywords

Neutrophils, Neisseria meningitidis, CEACAM1-binding recombinant polypeptide.

How to Cite 

Youssef, A.-R. (2021). Effect of Carcinoembryonic Antigen-related Cell Adhesion Molecule-binding Recombinant Polypeptide on the Killing of Neisseria meningitidis by Human Neutrophils. Journal of Umm Al-Qura University for Medical Sciences, 7(1), 6–9. 
https://doi.org/10.54940/ms10183116

License

 

جار التحميل