Saeed Kabrah^1*, Aiman Alsaegh¹, Fatimah Almahroqi¹, Arwa Flemban², Abdulrahman Mujalli¹, Ahmad A. Obaid¹, Amr J. Halawani¹, Waleed Bawazir^3,4, Malik A. Altayar⁵, Alhomidi Almotiri⁶, Ruba Omar Almaghrabi⁷, Ahmad F Arbaeen^1
1Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia
²Pathology Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
³Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
⁴Hematology Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia
⁵Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, Saudi Arabia
⁶Department of Clinical Laboratory Sciences, College of Applied Medical Sciences-Dawadmi, Shaqra University, Shaqra, Saudi Arabia
⁷Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Kingdom of Saudi Arabia
Volume: 10 | Issue: 2 | Pages: 1-10 | December 2024-|-https://doi.org/10.54940/ms71839391-|-PDF
Received:-20/10/2023-|-Received:-30/11/2023-|-Accepted:-11/12/2023
*Corresponding Author: smkabrah@uqu.edu.sa

Abstract
Background:-This study investigates the association between the ABO blood group genes and acute myeloid leukaemia (AML), a cancer characterized by rapid growth of abnormal white blood cells. Prior research indicates a potential link between blood group antigens and various cancers, including leukaemia. Our objective is to assess and validate changes in ABO gene expression in AML tissues compared to normal tissues, clarifying the extent of its association with AML pathogenesis and progression.
Methods:-In-silico analysis using platforms like GeneCards, HPA, ARChS4, BioGPS, and GEPIA2 was conducted to gather detailed data on ABO gene localization and its expression at RNA, mRNA, and protein levels in normal. And AML patients, aiding in understanding gene functionality.
Results:-The ABO gene was expressed in almost all subcellular components and throughout normal body tissues, including the myeloid tissues. A statistically significant association was found between the ABO gene expression and AML conditions in which the expression of the ABO gene is significantly suppressed in AML. There was worse survival for the AML patient with higher ABO expression without significant statistical evidence.
Conclusion:-The results reveal a reverse association between ABO gene expression and AML, highlighting the need for further research to elucidate ABO's role in AML progression.

Keywords
ABO gene, Acute myeloid leukemia, Tissue-specific expression, Cell-specific expression, In-silico analysis.

How to Cite
Kabrah, S., Alsaegh, A., Almahroqi, F., Flemban, A., Mujalli, A., Obaid, A. A., Halawani, A. J., Bawazir, W., Altayar, M. A., Almotiri, A., Almaghrabi, R. O., & Arbaeen, A. F. (2024). Validation of ABO Gene Expression in Normal Tissues and Acute Myeloid Leukaemia Using In-Silico Approaches,-Journal of Umm Al-Qura University for Medical Sciences, 10(2), 1-10.-https://doi.org/10.54940/ms71839391

License
1658-4740/© 2024-by the Authors. Published by-J. Umm Al-Qura Univ. Med. Sci.-This is an open-access article distributed under the terms and conditions of the-https://creativecommons.org/licenses/by-nc/4.0/-