Umm Al-Qura University

Umm Al-Qura University

Jamoum Department of Biology Conducts a Training Workshop on the Enzyme-Linked Immunosorbent Assay (ELISA) Device


Department News
Added on - 2019/12/14  |  اخر تعديل - 2019/12/14

For two days, the Department of Biology at Jamoum held a training workshop on the ELISA device, which has recently been procured at the Central Laboratory for Research and Postgraduate Studies. Over the course of two days—Tuesday and Wednesday, 19–20 November 2019—the Engineer Abdullah Muhammad gave practical training on how to use the device. This training took place in the presence of the Head of the Department of Biology, Dr. Samir bin Hasan Qari, and a select group of the department staff members, postgraduate students, and some interested colleagues.

The ELISA technique is based on the Enzyme Immunoassay EIA principle. In this technique, antibodies or antigens are attached to the holes in a plastic plate called a microtiter plate. Experimental and control samples are placed in the holes of the microtiter plate, and an association between antibodies and antigens is established.

And after an hour of incubation at room temperature, the plate is washed with a special washing solution to remove unrelated materials. An enzyme conjugate antibody is then added, and the plate is incubated for half an hour. After another washing procedure, the substrate is added and the plate is incubated for 20 minutes; the color (usually blue) then appears in the holes. Next, a stop solution is added, and the color changes from blue to yellow. The color intensity is measured using a 450 nm wavelength spectrometer. The antibody concentration or antigen to be measured is directly proportional to the intensity of the color.

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