Abstract

Diabetes mellitus is one of the most common diseases worldwide. Synthetic medicines usually have side effects; thus seeking alternatives is preferable. Ishige Okamurae Algae might act as a hypoglycemic agent beside its other bioactive effects. In the present study, the effect of IO on diabetic rats was studied concerning Omentin-1 gene, IL-6 gene, glucose, insulin, triglycerides, total cholesterol, LDL, HDL. Ishige Okamurae was extracted and fractionated. Twenty-four male Wistar rats were divided into 4 groups. Group 1 served as control, GRP 2 served as untreated diabetic group. GRP 3 non Diabatic  were administered with ethanol extract of IO at a dose of 250 mg/kg B.W , GRP 4 were diabetic and received an oral dose of 250 mg/kg Body wight (BW)  of ethanol extract of Ishige Okamurae.

At the end of the treatment period, animals were euthanized under anesthesia. Blood was withdrawn from the abdominal aorta and the adipose tissues were removed and stored at −80°C until use.

RNA was extracted from the frozen adipose tissues to study the effect of IO on both Omentin-1, and IL-6 gene. Relative expression in omentin and lL-6  mRNA levels were calculated. The current  study revealed that IO Algae plays a role in increasing blood insulin level, decreasing blood glucose, lowering cholesterol, decreasing triglycerides, decreasing LDL, increasing HDL, suppressing the expression of IL-6 gene, thus, reducing the harmful prolonged inflammatory effect of IL-6, whereas increasing the  expression of omentin-1 gene increases sensitivity to insulin. Therefore, it may be recommended to use this algae to treat diabetes and reduce its complications.

Keywords:

Ishige Okamurae, Algae Diabetes, Omentin-1 Gene, IL-6 Gene

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